Aluminum hydroxide nanoparticles show a stronger vaccine adjuvant activity than traditional aluminum hydroxide microparticles.
Aluminum hydroxide nanoparticles show a stronger vaccine adjuvant activity than traditional aluminum hydroxide microparticles.
复制标题
DOI：
10.1016/j.jconrel.2013.10.032
复制
发表时间：
2014-01-10
期刊：
影响因子：
--
通讯作者：
Cui Z
中科院分区：
文献类型：
--
作者：
Li X;Aldayel AM;Cui Z
氢氧化铝在各种人类疫苗中用作疫苗佐剂。不幸的是，尽管氢氧化铝具有良好的安全性，但它只能微弱或适度地增强抗原特异性抗体应答。当分散在水溶液中时，氢氧化铝形成1-20 µm的颗粒。越来越多的证据表明，作为疫苗或抗原载体的约200 nm或小于200 nm的纳米颗粒比大微粒具有更有效的佐剂活性。在本研究中，我们合成了112 nm的氢氧化铝纳米粒子。使用卵清蛋白和炭疽杆菌保护性抗原蛋白作为模型抗原，我们发现吸附在氢氧化铝纳米颗粒上的蛋白质抗原比吸附在约9.3 μm的传统氢氧化铝微粒上的相同蛋白质抗原诱导更强的抗原特异性抗体应答。氢氧化铝纳米颗粒的有效佐剂活性可能与其更有效地促进抗原呈递细胞吸收吸附在其上的抗原的能力有关。最后，注射部位由氢氧化铝纳米颗粒诱导的局部炎症比由微粒诱导的炎症轻。简单地将传统氢氧化铝佐剂的粒径减小到纳米代表了一种新颖且有效的方法来改善其佐剂性。
Aluminum hydroxide is used as a vaccine adjuvant in various human vaccines. Unfortunately, despite its favorable safety profile, aluminum hydroxide can only weakly or moderately potentiate antigen-specific antibody responses. When dispersed in an aqueous solution, aluminum hydroxide forms particulates of 1–20 µm. There is increasing evidence that nanoparticles around or less than 200 nm as vaccine or antigen carriers have a more potent adjuvant activity than large microparticles. In the present study, we synthesized aluminum hydroxide nanoparticles of 112 nm. Using ovalbumin and Bacillus anthracis protective antigen protein as model antigens, we showed that protein antigens adsorbed on the aluminum hydroxide nanoparticles induced a stronger antigen-specific antibody response than the same protein antigens adsorbed on the traditional aluminum hydroxide microparticles of around 9.3 µm. The potent adjuvant activity of the aluminum hydroxide nanoparticles was likely related to their ability to more effectively facilitate the uptake of the antigens adsorbed on them by antigen-presenting cells. Finally, the local inflammation induced by aluminum hydroxide nanoparticles in the injection sites was milder than that induced by microparticles. Simply reducing the particle size of the traditional aluminum hydroxide adjuvant into nanometers represents a novel and effective approach to improve its adjuvanticity.
登录
查看更多内容
影响因子：
3.4
作者：
Iyer, S;HogenEsch, H;Hem, SL
通讯作者：
Hem, SL
影响因子：
5.5
作者：
Mendez, Ilia Z. Romero;Shi, Yi;Hem, Stanley L.
通讯作者：
Hem, Stanley L.
影响因子：
2.2
作者：
Lutz, MB;Kukutsch, N;Schuler, G
通讯作者：
Schuler, G
影响因子：
5.5
作者：
HogenEsch, H
通讯作者：
HogenEsch, H
影响因子：
5.5
作者：
Gutierro, I;Hernández, RM;Pedraz, JL
通讯作者：
Pedraz, JL