Lipidomic analysis of human plasma reveals ether-linked lipids that are elevated in morbidly obese humans compared to lean.
Lipidomic analysis of human plasma reveals ether-linked lipids that are elevated in morbidly obese humans compared to lean.
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DOI：
10.1186/1758-5996-5-24
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发表时间：
2013-05-14
影响因子：
4.8
通讯作者：
Miller BF
中科院分区：
文献类型：
--
作者：
Donovan EL;Pettine SM;Hickey MS;Hamilton KL;Miller BF
通过脂质组学分析探讨瘦身受试者和肥胖受试者血浆中脂类分布的差异，然后采用体外方法检测已鉴定的脂类在内皮细胞病理生理学中的作用。采集了15例病态肥胖者和13例对照组的血浆。从血浆中提取血脂，并使用LC/MS和MS/MS进行分析，以表征血脂谱并识别肥胖受试者中与瘦受试者相比升高的血脂。正交偏最小二乘判别分析(OPLS-DA)模型显示，与瘦肉组相比，肥胖组的血脂谱有显著差异。分析在OPLS-DA模型中驱动组分离的脂类，以及肥胖组显著升高的脂类，鉴定出一组感兴趣的醚联磷脂酰胆碱(PC)和磷脂酰乙醇胺(PE)脂类。用乙醚连接的磷脂酰乙醇胺处理人冠状动脉内皮细胞，诱导细胞黏附分子的表达，这是内皮细胞激活的标志。然而，氧化磷脂酰胆碱产物在体外可以诱导内皮细胞激活，在体内不同组之间没有显著差异。这些数据表明，乙醚相关的脂类在肥胖相关的血脂异常和血管疾病中起作用。
Lipidomic analysis was performed to explore differences in lipid profiles between plasma from lean and obese subjects, followed by in vitro methods to examine a role for the identified lipids in endothelial cell pathophysiology. Plasma was collected from 15 morbidly obese and 13 control subjects. Lipids were extracted from plasma and analyzed using LC/MS, and MS/MS to characterize lipid profiles and identify lipids that are elevated in obese subjects compared to lean. Orthogonal partial least squares-discriminant analysis (OPLS-DA) modelling showed that lipid profiles were significantly different in obese subjects compared to lean. Analysis of lipids that were driving group separation in the OPLS-DA model and that were significantly elevated in the obese group led to identification of a group of ether-linked phosphatidylcholine (PC) and phosphatidylethanolamine (PE) lipids of interest. Treatment of human coronary artery endothelial cells with the ether-linked phosphatidylethanolamine induced expression of cell adhesion molecules, a hallmark of endothelial cell activation. However, oxidized phosphatidylcholine products that can induce endothelial cell activation in vitro, were not significantly different between groups in vivo. These data suggest a role for ether-linked lipids in obesity associated dyslipidemia and vascular disease.
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DOI：
10.1016/j.trsl.2008.12.005
发表时间：
2009-04
期刊：
Translational research : the journal of laboratory and clinical medicine
影响因子：
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