Chondroitin Sulphate/Dermatan Sulphate Proteoglycans: Potential Regulators of Corneal Stem/Progenitor Cell Phenotype In Vitro.
Chondroitin Sulphate/Dermatan Sulphate Proteoglycans: Potential Regulators of Corneal Stem/Progenitor Cell Phenotype In Vitro.
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DOI：
10.3390/ijms24032095
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发表时间：
2023-01-20
影响因子：
5.6
通讯作者：
Quantock, Andrew J.
中科院分区：
文献类型：
--
作者：
Bains, Kiranjit K.;Ashworth, Sean;Koudouna, Elena;Young, Robert D.;Hughes, Clare E.;Quantock, Andrew J.
硫酸软骨素（CS）蛋白聚糖沿着其糖胺聚糖（GAG）链具有可变的硫酸基序，其与关节软骨的干细胞龛密切相关，其中它们被认为影响驻留干细胞的特性。在这里，我们研究了免疫组织化学分布的混合CS/硫酸皮肤素（DS）GAG在周围的成年鸡角膜，这是角膜的干细胞生态位在一些物种的位置，使用单克隆抗体，6C 3，识别硫酸基序特异性CS/DS GAG表位。这表明阳性标记仅限于上皮下角膜基质，以及巩膜内的邻近骨性结构，称为小骨。当在涂有富含6C 3的CS/DS的细胞培养皿上培养时，从胚胎鸡角膜中分离的角膜基质细胞（角膜细胞）形成圆形集落，需要几天时间才能达到融合。这些角膜基质细胞的流式细胞术分析显示，与未暴露于CS/DS的细胞的表达水平相比，指示性干细胞标志物连接蛋白43（Cx43）、配对盒6（PAX 6）、B淋巴瘤莫洛尼鼠白血病病毒插入区-1（Bmi-1）和C-X-C趋化因子受体4（CXCR 4）的表达水平发生变化，表明分化程度较低的表型。这些发现支持CS/DS促进角膜细胞中干细胞表型保留的观点，就像它在其他结缔组织中所做的那样。
Chondroitin sulphate (CS) proteoglycans with variable sulphation-motifs along their glycosaminoglycan (GAG) chains are closely associated with the stem cell niche of articular cartilage, where they are believed to influence the characteristics of the resident stem cells. Here, we investigated the immunohistochemical distribution of hybrid CS/dermatan sulphate (DS) GAGs in the periphery of the adult chicken cornea, which is the location of the cornea’s stem cell niche in a number of species, using a monoclonal antibody, 6C3, that recognises a sulphation motif-specific CS/DS GAG epitope. This revealed positive labelling that was restricted to the subepithelial corneal stroma, as well as nearby bony structures within the sclera, called ossicles. When cultivated on cell culture dishes coated with 6C3-rich CS/DS, corneal stromal cells (keratocytes) that had been isolated from embryonic chicken corneas formed circular colonies, which took several days to reach confluency. A flow cytometric analysis of these keratocytes revealed changes in their expression levels of the indicative stem cell markers, Connexin 43 (Cx43), Paired Box 6 (PAX6), B-lymphoma Moloney murine leukemia virus insertion region-1 (Bmi-1), and C-X-C Chemokine Receptor 4 (CXCR4) suggestive of a less-differentiated phenotype compared with expression levels in cells not exposed to CS/DS. These findings support the view that CS/DS promotes the retention of a stem cell phenotype in corneal cells, much as it has been proposed to do in other connective tissues.
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影响因子：
7.5
作者：
Hashmani K;Branch MJ;Sidney LE;Dhillon PS;Verma M;McIntosh OD;Hopkinson A;Dua HS
通讯作者：
Dua HS
影响因子：
3.4
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通讯作者：
Birk, David E.
影响因子：
5.2
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通讯作者：
Li, De-Quan
影响因子：
2.7
作者：
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通讯作者：
West, JD
影响因子：
4.4
作者：
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通讯作者：
Tseng, SCG