Optofluidic detection for cellular phenotyping.

Optofluidic detection for cellular phenotyping.
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用于细胞表型的Optofluidic检测。

DOI:
10.1039/c2lc40509a
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发表时间:
2012-10-07
期刊:
影响因子:
6.1
通讯作者:
Kurabayashi K
Kurabayashi K
中科院分区:
工程技术1区
文献类型:
--
作者:
Tung YC;Huang NT;Oh BR;Patra B;Pan CC;Qiu T;Chu PK;Zhang W;Kurabayashi K

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对单个细胞内过程和分子相互作用的输出进行定量分析,对于推进准确的疾病筛查和个性化医疗至关重要。光学检测是生物和临床测定中最广泛适用的测量方法之一,并且用于细胞表型分析。近年来,微流体技术由于具有样品和试剂体积小、通量高以及在空间和时间域中精确的流动控制等优点而受到越来越多的关注。光流体学是将光学与微流体相结合的尝试,它在实现具有高精度、灵敏度、特异性和简单性的芯片上表型测量方面显示出巨大的潜力。本文综述了近年来用于细胞表型光学检测的光流控技术的最新进展。
Quantitative analysis of the output of processes and molecular interactions within a single cell is highly critical to the advancement of accurate disease screening and personalized medicine. Optical detection is one of the most broadly adapted measurement methods in biological and clinical assays and serves cellular phenotyping. Recently, microfluidics has obtained increasing attention due to several advantages, such as small sample and reagent volumes, very high throughput, and accurate flow control in the spatial and temporal domains. Optofluidics, which is the attempt to integrate optics with microfluidic, shows great promise to enable on-chip phenotypic measurements with high precision, sensitivity, specificity, and simplicity. This paper reviews the most recent developments of optofluidic technologies for cellular phenotyping optical detection.
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影响因子: 120.7
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