PUF-8 Functions Redundantly with GLD-1 to Promote the Meiotic Progression of Spermatocytes in Caenorhabditis elegans
PUF-8 Functions Redundantly with GLD-1 to Promote the Meiotic Progression of Spermatocytes in Caenorhabditis elegans
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PUF-8 与 GLD-1 冗余发挥作用，促进秀丽隐杆线虫精母细胞减数分裂进展
DOI：
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
K. Subramaniam
中科院分区：
文献类型：
--
作者：
Agarwal Priti;K. Subramaniam
生殖细胞减数分裂的顺利进行对配子发生至关重要。这一过程中的缺陷会影响正常的遗传传递，有时会导致种系肿瘤的形成。在秀丽隐杆线虫中，rna结合蛋白GLD-1对卵母细胞的减数分裂发育至关重要。然而，它在精子发生过程中的作用尚不清楚。在这里，我们发现GLD-1与PUF家族蛋白PUF-8一起发挥冗余功能，以确保精母细胞的正常减数分裂发育。当在20°c（秀丽隐杆线虫生长的标准实验室温度）下生长时，gld-1和puf-8单突变雄性和雌雄同体的初级精母细胞都能正常完成减数分裂。相比之下，一些gld-1；在两性中，Puf-8双突变精子细胞退出减数分裂并形成生殖细胞肿瘤。幼虫发育过程中，gld-1；puf-8双突变生殖细胞开始表达减数分裂标记物hm -3，失去P颗粒，形成精子特异性膜质细胞器，这些都是发育中的精子细胞的特征。然而，其中一些细胞迅速失去HIM-3，形成缺乏膜细胞器但含有P颗粒的生殖细胞肿瘤。在粗粒期晚期或糖尿病期阻断减数分裂进程的突变不能阻止肿瘤的发生，这表明gld-1；Puf-8双突变精母细胞在粗线期完成之前退出减数分裂。总之，本文的研究结果揭示了gld-1在雌雄同体和雄性精母细胞减数分裂发育中的新功能。
Successful meiotic progression of germ cells is crucial for gametogenesis. Defects in this process affect proper genetic transmission and sometimes lead to tumor formation in the germline. In Caenorhabditis elegans, the RNA-binding protein GLD-1 is essential for the meiotic development of oocytes. However, its role during spermatogenesis has not been understood. Here, we show that GLD-1 functions redundantly with the PUF family protein PUF-8 to ensure proper meiotic development of spermatocytes. When grown at 20°—the standard laboratory temperature for C. elegans growth—primary spermatocytes in both gld-1 and puf-8 single-mutant males and hermaphrodites complete the meiotic divisions normally. By contrast, some of the gld-1; puf-8 double-mutant spermatocytes exit meiosis and form germ cell tumors in both sexes. During larval development, gld-1; puf-8 double-mutant germ cells begin to express the meiotic marker HIM-3, lose P granules, and form the sperm-specific membranous organelle, which are characteristics of developing spermatocytes. However, some of these cells quickly lose HIM-3 and form germ cell tumors that lack membranous organelle but contain P granules. Mutations that block meiotic progression at late pachytene or diakinetic stage fail to arrest the tumorigenesis, suggesting that the gld-1; puf-8 double-mutant spermatocytes exit meiosis prior to the completion of pachytene. Together, results presented here uncover a novel function for gld-1 in the meiotic development of spermatocytes in both hermaphrodites and males.
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影响因子：
10.5
作者：
M. Zetka;I. Kawasaki;S. Strome;F. Müller
通讯作者：
M. Zetka;I. Kawasaki;S. Strome;F. Müller
影响因子：
3.3
作者：
Ross Francis;M. Barton;J. Kimble;T. Schedl
通讯作者：
Ross Francis;M. Barton;J. Kimble;T. Schedl
fem-3（秀丽隐杆线虫性别决定基因）的功能获得突变。
影响因子：
3.3
作者：
Barton,MK;Schedl,TB;Kimble,J
通讯作者：
Kimble,J
DOI：
10.1073/pnas.0509260103
发表时间：
2006-03-21
影响因子：
11.1
作者：
Gerber, AP;Luschnig, S;Herschlag, D
通讯作者：
Herschlag, D
影响因子：
2.7
作者：
Bernard, F.;Dutriaux, A.;Lalouette, A.
通讯作者：
Lalouette, A.