A Revised View on the Role of Surface AMPAR Mobility in Tuning Synaptic Transmission: Limitations, Tools, and Alternative Views.
A Revised View on the Role of Surface AMPAR Mobility in Tuning Synaptic Transmission: Limitations, Tools, and Alternative Views.
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DOI：
10.3389/fnsyn.2018.00021
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发表时间：
2018
影响因子：
3.7
通讯作者：
Selvin PR
中科院分区：
文献类型：
--
作者：
Delgado JY;Selvin PR
突触前终末的钙动力学调节大多数中枢兴奋性突触的反应动力学。然而，突触后alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic酸性亚型谷氨酸受体的迁移性在调节快速兴奋性突触传递中发挥作用的假说挑战了这一学说。在这篇综述中，我们重新评估了调节突触后AMPAR迁移率的因素，重新评估了模型参数，分析了实验工具，并根据最近的结果提供了替代的想法。特别是，用活神经元中的小荧光团标记AMPAR的新方法，结合超分辨率显微镜和亚秒动力学，支持了AMPAR主要位于突触内的观点，受到很大的限制，而且移动速度比之前认为的要慢得多。我们讨论了可能有必要重新研究突触后AMPAR移动性在调节快速兴奋性突触传递中的作用的新实验。
Calcium dynamics in presynaptic terminals regulate the response dynamics of most central excitatory synapses. However, this dogma has been challenged by the hypothesis that mobility of the postsynaptic alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid subtype glutamate receptors (AMPAR) plays a role in tuning fast excitatory synaptic transmission. In this review, we reevaluate the factors regulating postsynaptic AMPAR mobility, reassess the modeling parameters, analyze the experimental tools, and end by providing alternative ideas stemming from recent results. In particular, newer methods of labeling AMPARs with small fluorophores in live neurons, combined with super-resolution microscopy and sub-second dynamics, lends support to the idea that AMPARs are primarily within the synapse, are greatly constrained, and have much slower mobility than previously thought. We discuss new experiments which may be necessary to readdress the role of postsynaptic AMPAR mobility in tuning fast excitatory synaptic transmission.
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DOI：
10.1523/jneurosci.2399-07.2007
发表时间：
2007-08-01
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
DiGregorio DA;Rothman JS;Nielsen TA;Silver RA
通讯作者：
Silver RA
影响因子：
3.7
作者：
Freche D;Pannasch U;Rouach N;Holcman D
通讯作者：
Holcman D
影响因子：
25
作者：
Groc, L;Heine, M;Choquet, D
通讯作者：
Choquet, D
影响因子：
4.3
作者：
Belleudi, Francesca;Marra, Emanuele;Ciliberto, Gennaro
通讯作者：
Ciliberto, Gennaro
影响因子：
5.3
作者：
Groc, Laurent;Lafourcade, Mathieu;Cognet, Laurent
通讯作者：
Cognet, Laurent