Micro RNA profiling for the detection and differentiation of body fluids in forensic stain analysis
Micro RNA profiling for the detection and differentiation of body fluids in forensic stain analysis
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用于法医染色分析中体液检测和区分的 Micro RNA 分析
DOI：
10.1016/j.fsigss.2013.10.111
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发表时间：
2013
影响因子：
--
通讯作者：
Lin Zhang
中科院分区：
文献类型：
--
作者：
Bing Long;Kuanlin Liu;Weibo Liang;Lin Zhang
micro-RNA（miRNAs）是一类长度为18-24个核苷酸的非编码RNA（ncRNA）小分子，对许多细胞过程起着重要的调控作用。在过去的几年中，一些研究显示出兴趣的体液/组织通过miRNA谱鉴定。我们收集了四种体液样本，即血迹、精液、唾液和阴道分泌物。通过SYBR qPCR选择了一组八种miRNA标记物，即miR-451、miR-16、miR-135 b、miR-10 b、miR-658、miR-205、miR-124和miR-372进行分析。所有数据均针对U6 B标准化。我们通过二维miRNA分析进行了分析，并为不同种类的体液开发了四种NJ树，可用于识别未知体液。与其他研究相比，我们的结果与Hanson和Madea使用SYBR方法的研究结果没有差异，但与Zubakov和Hou使用Taqman方法的研究结果不相同。这些差异的原因可能是Taqman检测的敏感性和特异性更高。尽管我们的研究结果显示SYBR测定具有良好的重现性。
Micro-RNAs (miRNAs) are a class of small non-coding RNA (ncRNA) molecules with a length of 18–24 nucleotides which play an essential regulative role for many cellular processes. In the past few years, some researches show interests of body fluid/tissue identification through miRNA profiling. We collected four kinds of body fluid samples which are the stains of blood, semen, saliva and vaginal secretions. A panel of eight miRNA markers, which are miR-451, miR-16, miR-135b, miR-10b, miR-658, miR-205, miR-124, and miR-372, were selected to be analyzed through SYBR qPCR. All the data were normalized against U6B. We analyzed through two-dimensional miRNA assays and also developed four NJ trees for different kinds of body fluids, which can be used to identify unknown body fluid. Compared with other researches, our results show no difference with Hanson's and Madea's researches which using SYBR method, but are not as same as Zubakov and Hou's researches which using the Taqman method. Maybe the reason of these differences is the much higher sensitivity and specificity of Taqman assay. Even though our result of research shows good reproducibility of the SYBR assay.
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影响因子：
14.9
作者：
Chen C;Ridzon DA;Broomer AJ;Zhou Z;Lee DH;Nguyen JT;Barbisin M;Xu NL;Mahuvakar VR;Andersen MR;Lao KQ;Livak KJ;Guegler KJ
通讯作者：
Guegler KJ
影响因子：
1.6
作者：
Courts, Cornelius;Madea, Burkhard
通讯作者：
Madea, Burkhard
DOI：
10.1016/j.fsigss.2009.08.184
发表时间：
2009-12-01
期刊：
FORENSIC SCIENCE INTERNATIONAL GENETICS SUPPLEMENT SERIES
影响因子：
--
作者：
Hanson, E.;Lubenow, H.;Ballantyne, J.
通讯作者：
Ballantyne, J.
影响因子：
2.2
作者：
Juusola, J;Ballantyne, J
通讯作者：
Ballantyne, J
影响因子：
2.1
作者：
Zubakov D;Boersma AW;Choi Y;van Kuijk PF;Wiemer EA;Kayser M
通讯作者：
Kayser M