Differential expression of heat shock protein 90, 70, 60 in chicken muscles postmortem and its relationship with meat quality.

Differential expression of heat shock protein 90, 70, 60 in chicken muscles postmortem and its relationship with meat quality.
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鸡宰后肌肉中热休克蛋白90、70、60的差异表达及其与肉品质的关系

DOI:
10.5713/ajas.16.0132
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发表时间:
2017-01
期刊:
Asian-Australasian journal of animal sciences
影响因子:
--
通讯作者:
Li P
Li P
中科院分区:
其他
文献类型:
--
作者:
Zhang M;Wang D;Geng Z;Sun C;Bian H;Xu W;Zhu Y;Li P

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本研究旨在探究热休克蛋白(HSP)90、70和60在鸡肌肉中的表达情况以及它们与肉品质性状可能存在的关系。 对100只肉鸡的胸肌进行了滴水损失及其他品质参数分析,并通过免疫印迹法测定了热休克蛋白(HSP)90、70和60的水平。 根据数据,将鸡胸肌分为低(滴水损失≤5%)、中(5% <滴水损失< 9.5%)和高(滴水损失≥9.5%)滴水损失组。与低和中滴水损失组相比,高滴水损失组中HSP90和HSP60的表达显著较低(p < 0.05),而所有组中HSP70的含量相当(p > 0.05)。 本研究结果表明,较高水平的HSP90和HSP60可能有利于维持细胞功能和减少水分流失,并且它们可作为肉更好保水能力的潜在指标。
The aim of this study was to investigate the expression of heat shock protein (HSP) 90, 70, and 60 in chicken muscles and their possible relationship with quality traits of meat. The breast muscles from one hundred broiler chickens were analyzed for drip loss and other quality parameters, and the levels of heat shock protein (HSP) 90, 70, and 60 were determined by immunoblots. Based on the data, chicken breast muscles were segregated into low (drip loss≤5%), intermediate (5%<drip loss<9.5%) and high (drip loss≥9.5) drip loss groups. The expression of HSP90 and HSP60 were significantly lower in the high drip loss group compared to that in the low and intermediate drip loss group (p<0.05), while HSP70 was equivalent in abundance in all groups (p>0.05). Results of this study suggests that higher levels of HSP90 and HSP60 may be advantageous for maintenance of cell function and reduction of water loss, and they could act as potential indicator for better water holding capacity of meat.
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