Differential expression of heat shock transcription factors and heat shock proteins after acute and chronic heat stress in laying chickens (Gallus gallus).
Differential expression of heat shock transcription factors and heat shock proteins after acute and chronic heat stress in laying chickens (Gallus gallus).
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DOI：
10.1371/journal.pone.0102204
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Luo X
中科院分区：
文献类型：
--
作者：
Xie J;Tang L;Lu L;Zhang L;Xi L;Liu HC;Odle J;Luo X
高环境温度引起的热应激会对动物的表现产生负面影响。为了更好地了解热应激的生物学影响，对蛋鸡进行急性（24 小时内温度从 21°C 逐步升高至 35°C）或慢性（32°C，持续 8 周）高温暴露。高温挑战实验鸟体温显着升高（P<0.05）。然而，脂质和蛋白质的氧化状态以及热休克转录因子（HSF）和热休克蛋白（HSP）70和90的表达受到急性和慢性治疗的不同影响。在心脏、肝脏和肌肉中也发现了对热挑战的组织特异性反应。在心脏中，急性热挑战影响脂质氧化（P = 0.05）并且所有4种HSF基因表达均上调（P<0.05）。慢性热处理过程中，HSP 70 mRNA水平升高（P<0.05），HSP 90 mRNA水平降低（P<0.05）。在肝脏中，急性热应激期间蛋白质氧化减轻（P<0.05），但HSF2、3和4以及HSP 70基因表达被高度诱导（P<0.05）。慢性热处理使HSP90表达增加（P<0.05）。在肌肉中，两种类型的热应激都会增加蛋白质氧化，但 HSF 和 HSP 基因表达保持不变。急性热应激后，仅观察到 HSP 70 (P = 0.052) 和 90 (P = 0.054) 基因表达有增加的趋势。蛋鸡不同组织中 HSF 和 HSP 基因的差异表达表明，心脏可能更深层地激发抗热应激机制，而在热应激期间，肌肉受到的保护最少。除了 HSP 之外，HSF 基因表达也可以用作急性热应激期间的标志物。
Heat stress due to high environmental temperature negatively influences animal performances. To better understand the biological impact of heat stress, laying broiler breeder chickens were subjected either to acute (step-wisely increasing temperature from 21 to 35°C within 24 hours) or chronic (32°C for 8 weeks) high temperature exposure. High temperature challenges significantly elevated body temperature of experimental birds (P<0.05). However, oxidation status of lipid and protein and expression of heat shock transcription factors (HSFs) and heat shock proteins (HSPs) 70 and 90 were differently affected by acute and chronic treatment. Tissue-specific responses to thermal challenge were also found among heart, liver and muscle. In the heart, acute heat challenge affected lipid oxidation (P = 0.05) and gene expression of all 4 HSF gene expression was upregulated (P<0.05). During chronic heat treatment, the HSP 70 mRNA level was increased (P<0.05) and HSP 90 mRNA (P<0.05) was decreased. In the liver, oxidation of protein was alleviated during acute heat challenge (P<0.05), however, gene expression HSF2, 3 and 4 and HSP 70 were highly induced (P<0.05). HSP90 expression was increased by chronic thermal treatment (P<0.05). In the muscle, both types of heat stress increased protein oxidation, but HSFs and HSPs gene expression remained unaltered. Only tendencies to increase were observed in HSP 70 (P = 0.052) and 90 (P = 0.054) gene expression after acute heat stress. The differential expressions of HSF and HSP genes in different tissues of laying broiler breeder chickens suggested that anti-heat stress mechanisms might be provoked more profoundly in the heart, by which the muscle was least protected during heat stress. In addition to HSP, HSFs gene expression could be used as a marker during acute heat stress.
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影响因子：
3.7
作者：
Kikusato M;Toyomizu M
通讯作者：
Toyomizu M
影响因子：
8.8
作者：
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DOI：
10.1046/j.1432-1327.1999.00762.x
发表时间：
1999-10-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
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DOI：
10.1016/j.cbpa.2006.01.032
发表时间：
2006-05-01
影响因子：
2.3
作者：
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影响因子：
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作者：
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