Development of oral epithelial cell line ROE2 with differentiation potential from transgenic rats harboring temperature-sensitive simian virus40 large T-antigen gene.
Development of oral epithelial cell line ROE2 with differentiation potential from transgenic rats harboring temperature-sensitive simian virus40 large T-antigen gene.
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从携带温度敏感猿病毒40大T抗原基因的转基因大鼠中开发具有分化潜力的口腔上皮细胞系ROE2。
DOI：
10.1538/expanim.63.31
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发表时间：
2014
影响因子：
2.4
通讯作者：
Suzuki Y
中科院分区：
文献类型：
--
作者：
Tabuchi Y;Wada S;Ikegame M;Kariya A;Furusawa Y;Hoshi N;Yunoki T;Suzuki N;Takasaki I;Kondo T;Suzuki Y
我们已经开发了一个永生化的口腔上皮细胞系，ROE 2，从胎鼠携带温度敏感的猿猴病毒40大T抗原基因。细胞在33°C的容许温度或37°C的中间温度下连续生长。另一方面，在非允许温度39°C下，生长显著下降，细胞周期的Sub-G1期增加，表明细胞在非允许温度下发生凋亡。组织学和免疫细胞化学分析显示，ROE 2细胞在37°C下具有分层上皮样形态，并表达细胞角蛋白Krt 4和Krt 13，口腔非角化上皮细胞的标志蛋白。全球规模的综合微阵列分析，加上生物信息学工具，证明了一个重要的基因网络，从上调的基因。该基因网络包含16个基因，包括Cdkn 1a、Fos、Krt 13和Prdm 1，并且在生物学功能器官发育类别中主要与皮肤发育的生物学过程相关。这四个基因通过实时定量聚合酶链反应进行验证，结果与芯片数据基本一致。因此，预期该细胞系将可用作研究如生理功能以及口腔上皮细胞中基因表达的体外模型。
We have developed an immortalized oral epithelial cell line, ROE2, from fetal transgenic rats harboring temperature-sensitive simian virus 40 large T-antigen gene. The cells grew continuously at either a permissive temperature of 33°C or an intermediate temperature of 37°C. At the nonpermissive temperature of 39°C, on the other hand, growth decreased significantly, and the Sub-G1 phase of the cell cycle increased, indicating that the cells undergo apoptosis at a nonpermissive temperature. Histological and immunocytochemical analyses revealed that ROE2 cells at 37°C had a stratified epithelial-like morphology and expressed cytokeratins Krt4 and Krt13, marker proteins for oral nonkeratinized epithelial cells. Global-scale comprehensive microarray analysis, coupled with bioinformatics tools, demonstrated a significant gene network that was obtained from the upregulated genes. The gene network contained 16 genes, including Cdkn1a, Fos, Krt13, and Prdm1, and was associated mainly with the biological process of skin development in the category of biological functions, organ development. These four genes were validated by quantitative real-time polymerase chain reaction, and the results were nearly consistent with the microarray data. It is therefore anticipated that this cell line will be useful as an in vitro model for studies such as physiological functions, as well as for gene expression in oral epithelial cells.
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影响因子：
3.1
作者：
Ahmed, Kanwal;Furusawa, Yukihiro;Kadowaki, Makoto
通讯作者：
Kadowaki, Makoto
影响因子：
3.3
作者：
Alaminos, M.;Garzon, I.;Campos, A.
通讯作者：
Campos, A.
影响因子：
4.3
作者：
Neise, Denise;Sohn, Dennis;Jaenicke, Reiner U.
通讯作者：
Jaenicke, Reiner U.
影响因子：
4.4
作者：
Champliaud, MF;Baden, HP;Viel, A
通讯作者：
Viel, A
影响因子：
3
作者：
Gentile, Vittorio;Cooper, Arthur J. L.
通讯作者：
Cooper, Arthur J. L.