Highly sensitive immunoassays based on use of liposomes without complement.
Highly sensitive immunoassays based on use of liposomes without complement.
复制标题
基于使用不含补体的脂质体的高灵敏度免疫测定。
DOI：
10.1093/clinchem/30.9.1441
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发表时间：
1984
影响因子：
9.3
通讯作者：
M. Adamich
中科院分区：
文献类型：
--
作者：
W. J. Litchfield;J. Freytag;M. Adamich
我们描述了一种新的基于脂质体的免疫测定，其中使用共价连接的半抗原-溶细胞素缀合物代替补体和表面固定的免疫试剂。通过透析胆固醇和蛋黄卵磷脂悬浮液中的辛基葡萄糖苷，制备了稳定的单层脂质体，其中包封了碱性磷酸酶作为标记酶。所得到的囊泡可以通过加入蜂毒蜂毒肽或半抗原-蜂毒肽缀合物立即裂解。使用哇巴因，地高辛的类似物，我们合成的共轭物比单独的蜂毒肽更具溶解性，但在抗体的存在下被抑制。通过加入不同浓度的竞争性游离地高辛来影响这种抑制作用，以获得标准曲线。生物素-蜂毒肽偶联物可裂解相同的脂质体制剂，而生物素-蜂毒肽偶联物可被抗生物素蛋白抑制。后一种系统受游离生物素的影响，可用于将这种方法与各种异源免疫测定法结合。
We describe a novel liposome-based immunoassay in which covalently linked hapten-cytolysin conjugates are used instead of complement and surface-immobilized immunoreagents. Stable, unilamellar liposomes containing entrapped alkaline phosphatase as a marker enzyme were prepared by dialysis of octyl glucoside from suspensions of cholesterol and egg yolk lecithin. The resulting vesicles could be immediately lysed by addition of either bee venom melittin or hapten-melittin conjugates. Using ouabain, an analog of digoxin, we synthesized conjugates that were more lytic than mellitin alone but that were inhibited in the presence of antibody. This inhibition was affected by adding competing free digoxin at various concentrations to obtain standard curves. The same liposome preparations could be lysed with a biotin-melittin conjugate, which was inhibited by avidin. The latter system was affected by free biotin and might be used to couple this approach to various heterogeneous immunoassays.
影响因子：
3.4
作者：
DEGRADO, WF;MUSSO, GF;KEZDY, FJ
通讯作者：
KEZDY, FJ
影响因子：
2.9
作者：
MIMMS, LT;ZAMPIGHI, G;REYNOLDS, JA
通讯作者：
REYNOLDS, JA
电免疫测定。
影响因子：
3.4
作者：
Connell,GR;Sanders,KM;Williams,RL
通讯作者：
Williams,RL