Epidemiology and RAPD-PCR typing of thermophilic campylobacters from children under five years and chickens in Morogoro Municipality, Tanzania.
Epidemiology and RAPD-PCR typing of thermophilic campylobacters from children under five years and chickens in Morogoro Municipality, Tanzania.
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DOI：
10.1186/s12879-016-2031-z
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发表时间：
2016-11-21
影响因子：
3.7
通讯作者：
Kazwala RR
中科院分区：
文献类型：
--
作者：
Chuma IS;Nonga HE;Mdegela RH;Kazwala RR
弯曲杆菌是弯曲菌科弯曲杆菌属的革兰氏阴性鞭毛菌。这些病原体会在人和动物群体中引起人畜共患传染病。这项研究是在2006年12月至2007年5月期间进行的，目的是确定坦桑尼亚莫罗戈罗市5岁以下儿童和鸡只中嗜热弯曲杆菌的流行率、风险因素和遗传多样性。采用Skirrow‘s方法从268份人粪便标本和419份鸡泄殖腔拭子中分离鉴定出弯曲杆菌。还收集了与人类感染相关的患者生物数据和风险因素。采用OPA 11引物(5‘-CAA、TCG、CCG、T-3’)的随机扩增技术，对不同菌株的遗传多样性进行了分析。用Bionumerics软件和目视检查进行系统发育分析和带型比较。对268例儿童粪便标本和419例鸡泄殖腔拭子进行了检测。儿童中嗜热弯曲杆菌的感染率为19%，男性(23.5%)高于女性(13.8%)(p = 0.046)。空肠弯曲杆菌(78.4%)的分离率(p = 0.000)高于大肠埃希菌(19.6%)，2%为未鉴定菌株。鸡群中空肠弯曲杆菌的感染率为42.5%，分离率(p = 0.000)为87%，高于大肠埃希菌(13%)。从土鸡中回收弯曲杆菌的频率较高(p = 0.000)，其次是公鸡(52.2%)、肉鸡(50.0%)，蛋鸡最低(22.7%)。同时饲养鸡只(p = 0.000)、鸡种(p = 0.000)和鸡群规模(p = 0.007)是鸡感染的危险因素。对152株嗜热弯曲菌进行了基因分型，其中空肠弯曲菌74株，人40株；大肠埃希菌38株，鸡28株，人10株。从儿童和鸡中分离的弯曲菌具有高度的多样性，仅有6.1%的空肠弯曲菌和5.3%的大肠埃希菌具有100%的遗传相似性。这项研究记录了莫罗戈罗市5岁以下儿童和鸡中嗜热弯曲杆菌的高流行率。从儿童和鸡分离到的少数空肠和大肠埃希氏菌的遗传相似性表明，这些病原体在5岁以下儿童和鸡之间存在交叉传播。
Campylobacter species are gram negative and flagellated bacteria under the genus Campylobacter, family Campylobacteriaceae. These pathogens cause zoonotic infections among human and animal populations. This study was undertaken between December 2006 and May 2007 to determine prevalence, risk factors and genetic diversity of thermophilic Campylobacter isolates from children less than 5 years and chickens in Morogoro Municipality, Tanzania. The Skirrow’s protocol was used for isolation and identification of Campylobacter from 268 human stool specimens and 419 chicken cloacal swabs. Patient biodata and risk factors associated with human infection were also collected. Genetic diversity of Campylobacter isolates was determined by a RAPD-PCR technique using OPA 11 primer (5′-CAA TCG CCG T-3′). Phylogenetic analysis and band pattern comparison were done by Bionumerics software and visual inspection. Stool samples from 268 children and 419 cloacal swabs from chickens were analyzed. Prevalence of thermophilic Campylobacters in children was 19% with higher isolation frequency (p = 0.046) in males (23.5%) than females (13.8%). Campylobacter jejuni (78.4%) was more isolated (p = 0.000) than C. coli (19.6%) and 2% were unidentified isolates. In chickens, the prevalence was 42.5% with higher isolation rate (p = 0.000) of C. jejuni (87%) than C. coli (13%). Campylobacters were more frequently recovered (p = 0.000) from indigenous/ local chickens (75.0%) followed by cockerels (52.2%), broilers (50.0%) and lowest in layers (22.7%). Keeping chickens without other domestic animals concurrently (p = 0.000), chicken types (p = 0.000) and flock size (p = 0.007) were risk factors for infection in chickens. One hundred and fifty two (152) thermophillic Campylobacter isolates were genotyped by RAPD-PCR of which 114 were C. jejuni (74 from chickens and 40 humans) and 38 C. coli (28 from chickens and 10 humans). Comparison of Campylobacter isolates from children and chickens revealed high diversity with only 6.1% of C. jejuni and 5.3% of C. coli being 100% genetically similar. This study has recorded high prevalence of thermophilic Campylobacter in children less than 5 years and chickens in Morogoro municipality. The observed genetic similarity among few C. jejuni and C. coli isolates from children and chicken suggests existence of cross transmission of these pathogens between children under 5 years and chickens.
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影响因子：
--
作者：
Lindblom, GB;Ahren, C;Temu, M
通讯作者：
Temu, M
影响因子：
1.7
作者：
Nonga, Hezron E.;Sells, P.;Karimuribo, E. D.
通讯作者：
Karimuribo, E. D.
影响因子：
2.6
作者：
HERNANDEZ, J;FAYOS, A;OWEN, RJ
通讯作者：
OWEN, RJ
影响因子：
3.7
作者：
Kusiluka, LJM;Karimuribo, ED;Kambarage, DM
通讯作者：
Kambarage, DM
DOI：
10.1111/j.1439-0450.2006.00926.x
发表时间：
2006-04-01
期刊：
JOURNAL OF VETERINARY MEDICINE SERIES B-INFECTIOUS DISEASES AND VETERINARY PUBLIC HEALTH
影响因子：
--
作者：
Mdegela, R. H.;Nonga, H. E.;Kazwala, R. R.
通讯作者：
Kazwala, R. R.