Separable transcriptional regulatory domains within Otd control photoreceptor terminal differentiation events.
Separable transcriptional regulatory domains within Otd control photoreceptor terminal differentiation events.
复制标题
DOI：
10.1016/j.ydbio.2010.08.016
复制
发表时间：
2010-11-01
影响因子：
2.7
通讯作者：
Cook TA
中科院分区：
文献类型：
--
作者：
McDonald EC;Xie B;Workman M;Charlton-Perkins M;Terrell DA;Reischl J;Wimmer EA;Gebelein BA;Cook TA
直齿体（Otd）相关的转录因子是必不可少的前图案和脑形态发生从刺胞动物，哺乳动物，和遗传基础的几个人类视网膜病变。尽管他们的关键发育功能，相对知之甚少，关于这些因素如何调节下游效应在细胞或组织特异性的方式的分子基础。许多无脊椎动物和脊椎动物编码两到三个Otd蛋白，而果蝇编码一个Otd蛋白。在果蝇视网膜中，Otd控制所有光感受器的横纹肌形态发生，并以光感受器亚型特异性方式调节不同的视紫红质编码基因。在这里，我们进行了结构-功能分析OTD在果蝇眼睛发育过程中使用体内救援实验和体外转录调控分析。我们的研究结果表明，OTD需要至少三个不同的转录调控结构域来控制感光细胞特异性视紫红质基因的表达和感光细胞的形态发生。我们的研究结果还揭示了Otd在阻止蓝色与绿色敏感的R8光感受器中感觉受体共表达方面的一个先前未知的作用。序列分析表明，许多转录调控结构域确定在这里是保守的多双翅目OTD相关蛋白。因此，这些研究提供了一个基础，以确定参与广泛的发展过程中的共享分子途径。
Orthodenticle (Otd)-related transcription factors are essential for anterior patterning and brain morphogenesis from Cnidaria to Mammals, and genetically underlie several human retinal pathologies. Despite their key developmental functions, relatively little is known regarding the molecular basis of how these factors regulate downstream effectors in a cell- or tissue-specific manner. Many invertebrate and vertebrate species encode two to three Otd proteins, whereas Drosophila encodes a single Otd protein. In the fly retina, Otd controls rhabdomere morphogenesis of all photoreceptors and regulates distinct Rhodopsin-encoding genes in a photoreceptor subtype-specific manner. Here, we performed a structure-function analysis of Otd during Drosophila eye development using in vivo rescue experiments and in vitro transcriptional regulatory assays. Our findings indicate that Otd requires at least three distinct transcriptional regulatory domains to control photoreceptor-specific rhodopsin gene expression and photoreceptor morphogenesis. Our results also uncover a previously unknown role for Otd in preventing co-expression of sensory receptors in blue vs. green-sensitive R8 photoreceptors. Sequence analysis indicates that many of the transcriptional regulatory domains identified here are conserved in multiple Diptera Otd-related proteins. Thus, these studies provide a basis for identifying shared molecular pathways involved in a wide range of developmental processes.
登录
查看更多内容
影响因子：
64.8
作者：
COHEN, SM;JURGENS, G
通讯作者：
JURGENS, G
影响因子：
10.5
作者：
FORTINI, ME;RUBIN, GM
通讯作者：
RUBIN, GM
影响因子：
16.2
作者：
Chou, WH;Hall, KJ;Britt, SG
通讯作者：
Britt, SG
影响因子：
64.5
作者：
Furukawa, T;Morrow, EM;Cepko, CL
通讯作者：
Cepko, CL
影响因子：
9.2
作者：
Morante, Javier;Desplan, Claude
通讯作者：
Desplan, Claude