Outer brain barriers in rat and human development.
Outer brain barriers in rat and human development.
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DOI：
10.3389/fnins.2015.00075
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发表时间：
2015
影响因子：
4.3
通讯作者：
Møllgård K
中科院分区：
文献类型：
--
作者：
Brøchner CB;Holst CB;Møllgård K
大脑表面的复杂屏障，特别是在发育过程中，定义不清。在成人中，蛛网膜血-脑脊液（CSF）屏障通过紧密连接的细胞层将开窗硬脑膜血管与CSF分开。外脑脊液-脑屏障通过覆盖脑脊液面层的初始径向胶质端足层在脑和蛛网膜下腔脑脊液之间提供扩散限制。为了进一步表征这些界面，我们使用免疫组织化学和共聚焦显微镜检查了E10至P0的胚胎大鼠脑，以及人类胚胎和胎儿（怀孕后6 - 21周）和成人的前脑。针对cladin -11、BLBP、collagen 1、SSEA-4、MAP2、YKL-40及其受体IL-13Rα2和EAAT1的抗体被用来描述脑外屏障的形态特征和功能方面。Claudin-11是蛛网膜血-脑脊液屏障的可靠标志物。胶原蛋白1勾勒出蛛网膜下腔，并染色枕状面层。BLBP定义了径向胶质终足层，SSEA-4和YKL-40均存在于小脑膜细胞和终足层，并转化为胶质限制物。IL-13Rα2和EAAT1存在于终足层，说明转运蛋白/受体存在于csf -脑外屏障中。成人脑MAP2免疫染色勾勒出胶质细胞边界的下边界；部分地区尾足残体YKL-40阳性。我们认为脑外屏障至少由3个界面组成：横跨蛛网膜屏障细胞层的血-脑脊液屏障、横跨脑脊液微血管的血-脑脊液屏障和由胶质端足层/脑脊液面层组成的脑外屏障。
Complex barriers at the brain's surface, particularly in development, are poorly defined. In the adult, arachnoid blood-cerebrospinal fluid (CSF) barrier separates the fenestrated dural vessels from the CSF by means of a cell layer joined by tight junctions. Outer CSF-brain barrier provides diffusion restriction between brain and subarachnoid CSF through an initial radial glial end feet layer covered with a pial surface layer. To further characterize these interfaces we examined embryonic rat brains from E10 to P0 and forebrains from human embryos and fetuses (6–21st weeks post-conception) and adults using immunohistochemistry and confocal microscopy. Antibodies against claudin-11, BLBP, collagen 1, SSEA-4, MAP2, YKL-40, and its receptor IL-13Rα2 and EAAT1 were used to describe morphological characteristics and functional aspects of the outer brain barriers. Claudin-11 was a reliable marker of the arachnoid blood-CSF barrier. Collagen 1 delineated the subarachnoid space and stained pial surface layer. BLBP defined radial glial end feet layer and SSEA-4 and YKL-40 were present in both leptomeningeal cells and end feet layer, which transformed into glial limitans. IL-13Rα2 and EAAT1 were present in the end feet layer illustrating transporter/receptor presence in the outer CSF-brain barrier. MAP2 immunostaining in adult brain outlined the lower border of glia limitans; remnants of end feet were YKL-40 positive in some areas. We propose that outer brain barriers are composed of at least 3 interfaces: blood-CSF barrier across arachnoid barrier cell layer, blood-CSF barrier across pial microvessels, and outer CSF-brain barrier comprising glial end feet layer/pial surface layer.
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影响因子：
2.3
作者：
Chung, C;Tallerico, T;Seeman, P
通讯作者：
Seeman, P
影响因子：
3.2
作者：
Brochner, Christian B.;Johansen, Julia S.;Mollgard, Kjeld
通讯作者：
Mollgard, Kjeld
DOI：
10.1097/00005072-198803000-00009
发表时间：
1988-03-01
影响因子：
3.2
作者：
KADHIM, HJ;GADISSEUX, JF;EVRARD, P
通讯作者：
EVRARD, P
影响因子：
9.3
作者：
Colton CA;Mott RT;Sharpe H;Xu Q;Van Nostrand WE;Vitek MP
通讯作者：
Vitek MP
DOI：
10.1023/a:1018527928760
发表时间：
1997-03-01
期刊：
JOURNAL OF NEUROCYTOLOGY
影响因子：
--
作者：
Balslev, Y;Saunders, NR;Mollgard, K
通讯作者：
Mollgard, K