Host gene expression signatures discriminate between ferrets infected with genetically similar H1N1 strains.
Host gene expression signatures discriminate between ferrets infected with genetically similar H1N1 strains.
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宿主基因表达签名区分了被遗传上相似H1N1菌株感染的雪貂。
DOI：
10.1371/journal.pone.0040743
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Bruder CE
中科院分区：
文献类型：
--
作者：
Ljungberg K;McBrayer A;Camp JV;Chu YK;Tapp R;Noah DL;Grimes S;Proctor ML;Liljeström P;Jonsson CB;Bruder CE
不同的呼吸道病毒在宿主中诱导病毒特异性基因表达。最近的证据，包括本文提出的证据，表明流感病毒的遗传相关分离株在几种动物模型中诱导了株特异性宿主基因调节。在这里，我们确定了感染大流行性流感A/加州/07/2009、A/墨西哥/4482/2009或季节性流感A/布里斯班/59/2007的雪貂的系统性毒株特异性基因表达特征。使用不相关的收缩质心分类，我们能够准确地识别感染流感病毒株与10个选定的基因的组合基因表达谱，独立于疾病的严重程度。另一个基因签名由7个基因组成，可以根据肺部病理对样本进行分类。此外，我们确定了由31个探针组成的基因表达谱，可以根据菌株和疾病的严重程度对样品进行分类。因此，我们表明，基于表达的非感染组织的分析能够区分基因相关的流感病毒以及肺部病理。这些结果为开发流感诊断的替代工具打开了大门。
Different respiratory viruses induce virus-specific gene expression in the host. Recent evidence, including those presented here, suggests that genetically related isolates of influenza virus induce strain-specific host gene regulation in several animal models. Here, we identified systemic strain-specific gene expression signatures in ferrets infected with pandemic influenza A/California/07/2009, A/Mexico/4482/2009 or seasonal influenza A/Brisbane/59/2007. Using uncorrelated shrunken centroid classification, we were able to accurately identify the infecting influenza strain with a combined gene expression profile of 10 selected genes, independent of the severity of disease. Another gene signature, consisting of 7 genes, could classify samples based on lung pathology. Furthermore, we identified a gene expression profile consisting of 31 probes that could classify samples based on both strain and severity of disease. Thus, we show that expression-based analysis of non-infected tissue enables distinction between genetically related influenza viruses as well as lung pathology. These results open for development of alternative tools for influenza diagnostics.
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