Juvenile depletion of microglia reduces orientation but not high spatial frequency selectivity in mouse V1.
Juvenile depletion of microglia reduces orientation but not high spatial frequency selectivity in mouse V1.
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DOI：
10.1038/s41598-022-15503-0
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发表时间：
2022-07-27
影响因子：
4.6
通讯作者：
Gandhi, Sunil P.
中科院分区：
文献类型：
--
作者：
Velez, Dario X. Figueroa;Arreola, Miguel;Huh, Carey Y. L.;Green, Kim;Gandhi, Sunil P.
小胶质细胞包含多种机制，在出生后发育过程中形成突触景观。然而，小胶质细胞介导的突触变化是否反映了感觉皮层神经元反应的发育完善，仍然知之甚少。在出生后的生活中，初级视皮层（V1）神经元反应的方向和空间频率选择性增加的发展支持高视力的出现。在这里，我们使用集落刺激因子1受体（CSF 1 R）抑制剂PLX 5622快速，持久地消耗小胶质细胞的小鼠在幼年期，其中增加的方向和空间频率选择性出现。兴奋性和抑制性调谐性能同时测定使用多光子钙成像在层II/III的小鼠V1。我们发现，小胶质细胞耗竭一般增加诱发活动，这反过来又降低了方向选择性。令人惊讶的是，小胶质细胞不需要出现高空间频率调谐反应。此外，小胶质细胞耗竭没有扰乱皮质双眼，表明正常的深度处理。总之，我们的发现，V1的方向和高空间频率选择性的差异支持小胶质细胞揭示，小胶质细胞需要正常的感觉处理，虽然有选择性。
Microglia contain multiple mechanisms that shape the synaptic landscape during postnatal development. Whether the synaptic changes mediated by microglia reflect the developmental refinement of neuronal responses in sensory cortices, however, remains poorly understood. In postnatal life, the development of increased orientation and spatial frequency selectivity of neuronal responses in primary visual cortex (V1) supports the emergence of high visual acuity. Here, we used the colony-stimulating factor 1 receptor (CSF1R) inhibitor PLX5622 to rapidly and durably deplete microglia in mice during the juvenile period in which increased orientation and spatial frequency selectivity emerge. Excitatory and inhibitory tuning properties were measured simultaneously using multi-photon calcium imaging in layer II/III of mouse V1. We found that microglia depletion generally increased evoked activity which, in turn, reduced orientation selectivity. Surprisingly, microglia were not required for the emergence of high spatial frequency tuned responses. In addition, microglia depletion did not perturb cortical binocularity, suggesting normal depth processing. Together, our finding that orientation and high spatial frequency selectivity in V1 are differentially supported by microglia reveal that microglia are required normal sensory processing, albeit selectively.
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影响因子：
16.2
作者：
Cheadle L;Rivera SA;Phelps JS;Ennis KA;Stevens B;Burkly LC;Lee WA;Greenberg ME
通讯作者：
Greenberg ME
影响因子：
4.6
作者：
Acharya MM;Green KN;Allen BD;Najafi AR;Syage A;Minasyan H;Le MT;Kawashita T;Giedzinski E;Parihar VK;West BL;Baulch JE;Limoli CL
通讯作者：
Limoli CL
通过三重免疫染色鉴定小鼠视觉皮层中 GABA 能神经元的多种不同亚型。
影响因子：
2.9
作者：
Gonchar, Yuri;Wang, Quanxin;Burkhalter, Andreas
通讯作者：
Burkhalter, Andreas
影响因子：
16.2
作者：
Holtmaat, AJGD;Trachtenberg, JT;Svoboda, K
通讯作者：
Svoboda, K
影响因子：
5.5
作者：
GIFFIN, F;MITCHELL, DE
通讯作者：
MITCHELL, DE