Single-Laboratory Validation for the Determination of Cocoa Flavanols and Procyanidins (by Degree of Polymerization DP1-7) in Cocoa-Based Products by Hydrophilic Interaction Chromatography Coupled with Fluorescence Detection: First Action 2020.05.
Single-Laboratory Validation for the Determination of Cocoa Flavanols and Procyanidins (by Degree of Polymerization DP1-7) in Cocoa-Based Products by Hydrophilic Interaction Chromatography Coupled with Fluorescence Detection: First Action 2020.05.
复制标题
DOI：
10.1093/jaoacint/qsaa132
复制
发表时间：
2021-05-21
影响因子：
1.6
通讯作者：
Kwik-Uribe C
中科院分区：
文献类型：
--
作者：
Bussy U;Hewitt G;Olanrewaju Y;May BR;Anderson N;Ottaviani JI;Kwik-Uribe C
黄烷醇和原花青素是在许多食物中发现的复杂生物活性物质，如可可。由于可可黄烷醇和原花青素的消费与健康益处有关，因此它们正受到消费者、工业界、研究人员和监管机构越来越多的关注。本研究的目的是验证一种方法，使用亲水相互作用色谱法（HILIC）与荧光检测（FLD）和市售的参考材料，用于测定可可产品中的黄烷醇和原花青素（CF）。评价了CF含量范围为0.8至500 mg/g的可可基质的方法性能，包括低CF基质（牛奶和黑巧克力、可可粉和液体）和高CF基质（可可提取物和膳食补充剂产品）。通过测定各基质的灵敏度、选择性、线性、稳定性、耐用性、准确度和精密度，在单个实验室中对该方法进行了验证。对CF含量范围为0.8 - 500 mg/g的可可基质进行了方法验证。CF的准确度范围为86 - 99%，重复性（RSDr）范围为1.5 - 8.6%。通过对各种可可基质的单一实验室验证研究获得的分析性能表明，该方法适用于测定可可产品中的黄烷醇和原花青素。采用亲水作用色谱法（HILIC）结合荧光检测成功测定了多种产品类型中的总CF含量。单一实验室方法验证结果表明，该方法适用于含有<0.8至500 mg/g CF的可可基质。
Flavanols and procyanidins are complex bioactives found in many foods such as cocoa. As their consumption is associated with health benefits, cocoa flavanols and procyanidins are receiving increasing attention from consumers, industry, researchers, and regulators. The objective of this study is to validate a method using hydrophilic interaction chromatography (HILIC) with fluorescence detection (FLD) and a commercially available reference material for the determination of flavanols and procyanidins (CF) in cocoa-based products. Method performances were evaluated for cocoa matrices with CF content that ranged from 0.8 to 500 mg/g, which included low CF matrices (milk and dark chocolate, cocoa powder, and liquor) and high CF matrices (cocoa extract and dietary supplement products). The method was validated in a single-laboratory by determining sensitivity, selectivity, linearity, stability, robustness, accuracy, and precision for each of the matrices. The method was validated for cocoa matrices with CF content that ranged from 0.8 to 500 mg/g. Accuracy ranged from 86 to 99% and repeatability (RSDr) from 1.5 to 8.6% for CF. Analytical performances acquired through this single-laboratory validation study for a wide range of cocoa-based matrices demonstrate that this method is fit-for-purpose for the determination of flavanols and procyanidins in cocoa-based products. Hydrophilic interaction chromatography (HILIC) with fluorescence detection was successfully used to determine total CF content in multiple product types. Single-laboratory method validation results demonstrate that the method is fit for purpose for cocoa-based matrices containing <0.8 to 500 mg/g of CF.
登录
查看更多内容
DOI：
10.3945/ajcn.114.092189
发表时间：
2015-03
期刊：
The American journal of clinical nutrition
影响因子：
--
作者：
Mastroiacovo D;Kwik-Uribe C;Grassi D;Necozione S;Raffaele A;Pistacchio L;Righetti R;Bocale R;Lechiara MC;Marini C;Ferri C;Desideri G
通讯作者：
Desideri G
影响因子：
4.1
作者：
Hollands, Wendy J.;Voorspoels, Stefan;Kroon, Paul A.
通讯作者：
Kroon, Paul A.
影响因子：
25
作者：
Brickman AM;Khan UA;Provenzano FA;Yeung LK;Suzuki W;Schroeter H;Wall M;Sloan RP;Small SA
通讯作者：
Small SA
影响因子：
6.1
作者：
Lin LZ;Sun J;Chen P;Monagas MJ;Harnly JM
通讯作者：
Harnly JM
影响因子：
6.1
作者：
Lakenbrink, C;Engelhardt, UH;Wray, V
通讯作者：
Wray, V