Protein production by human gingival fibroblasts is enhanced by guanidine EDTA extracts of cementum.
Protein production by human gingival fibroblasts is enhanced by guanidine EDTA extracts of cementum.
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牙骨质的胍 EDTA 提取物可增强人牙龈成纤维细胞的蛋白质产生。
DOI：
10.1111/j.1600-0765.1987.tb01542.x
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发表时间：
1987
影响因子：
3.5
通讯作者：
Ft. A. Foster
中科院分区：
文献类型：
--
作者：
M. J. Somerman;S. Archer;A. Shteyer;Ft. A. Foster
将人牙龈成纤维细胞的非融合培养物暴露于牙骨质的胍和胍EDTA提取物48小时。为了比较牙骨质提取物对成纤维细胞与其他矿化组织提取物的影响，还将细胞暴露于牙本质和牙槽骨的胍和胍EDTA提取物。在最后24小时内对细胞进行放射性标记。通过放射性脯氨酸的掺入测量总蛋白质产量。通过用细菌胶原酶消化放射性蛋白质混合物来估计胶原蛋白的产生。与对照组相比，所有胍EDTA提取物引起总蛋白质产量的统计学显著增加。在50 μg/ml的提取物中，骨、牙骨质和牙本质的蛋白质产量分别增加340%、143%和338%。对于胶原蛋白生产获得了类似的结果。相反，胍提取物对人牙龈成纤维细胞的蛋白质或胶原蛋白的产生没有影响。这些数据表明，牙龈成纤维细胞的功能可以改变相关矿化组织的蛋白质。识别这些蛋白质并了解它们的生物学功能将增强我们对调节结缔组织再生机制的认识。
Nonconfluent cultures of human gingival fibroblasts were exposed to both guanidine and guanidine EDTA extracts of cementum for 48 hours. To compare the effects of cementum extracts on fibroblasts with other mineralized tissue extracts, cells were also exposed to guanidine and guanidine EDTA extracts of dentin and alveolar bone. The cells were radioactively labeled during the last 24 h. Total protein production was measured via the incorporation of radioactive proline. Collagen production was estimated by digestion of the radioactive protein mixture with bacterial collagenase. All guanidine EDTA extracts elicited statistically significant increases in total protein production compared to controls. At 50 μg/ml of extract, the increases in protein production were 340%, 143% and 338% for bone, cementum and dentin, respectively. Similar results were obtained for collagen production. In contrast, the guanidine extracts had no effect on either protein or collagen production by human gingival fibroblasts. These data indicate that the functions of gingival fibroblasts can be altered by proteins from associated mineralized tissues. Identifying such proteins and understanding their biological functions will enhance our knowledge of the mechanisms that regulate connective tissue regeneration.
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影响因子：
3
作者：
T. M. Hassell;E. Stanek
通讯作者：
T. M. Hassell;E. Stanek
骨提取物含有有效的骨形成调节剂。
DOI：
10.1016/0304-4165(82)90123-4
发表时间：
1982
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Drivdahl,RH;Howard,GA;Baylink,DJ
通讯作者：
Baylink,DJ
来自胚胎肌肉的间充质细胞响应于骨的细胞外基质成分而在体外转化为软骨。
DOI：
10.1073/pnas.81.11.3419
发表时间：
1984
影响因子：
11.1
作者：
Sampath,TK;Nathanson,MA;Reddi,AH
通讯作者：
Reddi,AH
对早发性牙周炎病因和发病机制的新认识。
影响因子：
4.3
作者：
Page,RC;Baab,DA
通讯作者：
Baab,DA