Development of a colorimetric and fluorescence dual-mode immunoassay for the precise identification of Alicyclobacillus acidoterrestris in apple juice
Development of a colorimetric and fluorescence dual-mode immunoassay for the precise identification of Alicyclobacillus acidoterrestris in apple juice
复制标题
开发比色荧光双模式免疫分析法精确鉴定苹果汁中的酸土脂环酸芽孢杆菌
DOI：
10.1016/j.foodcont.2021.107898
复制
发表时间：
2021-06
期刊：
影响因子：
6
通讯作者：
Yuan Yahong
中科院分区：
文献类型：
--
作者：
Shi Yiheng;Sun Yuhan;Qu Xiaowei;Zhou Lei;Wang Zhouli;Gao Zhenpeng;Hu Zhongqiu;Yue Tianli;Yuan Yahong
酸土菌是导致商品果汁变质的主要危害因素，可造成严重的经济损失。目前的检测方法需要高端设备，特异性和灵敏度较低。为了解决这些问题，我们在此描述了对A具有高特异性的单克隆抗体。酸土以细胞壁蛋白为抗原。基于氧化对苯二胺对荧光素的猝灭作用，建立了一种基于该抗体的比色和荧光双模式免疫分析系统。该方法可准确区分目标菌和其他菌种，检测限提高到4.8 × 102 CFU/mL。苹果汁被人工污染A. 1CFU/mL的酸土杆菌经24 h富集后可检测为阳性。该免疫检测平台为A的检测提供了一种快速、方便、灵敏、选择性强的方法。酸土生菌，作为果汁工业中防止腐败的强大工具具有巨大潜力。
Alicyclobacillus acidoterrestrisis the main hazardous factor leading to deterioration of commercialized fruit juices, as it can result in serious economic loss to the industry. Currently detection methods require high-end equipment with low specificity and sensitivity. To address these problems, we describe herein a monoclonal antibody with high specificity toA. acidoterrestrisusing a cell wall protein as antigen. A colorimetric and fluorescence dual-mode immunoassay system employing the prepared antibody was then developed based on the quenching effect of oxidizedp-phenylenediamine on fluorescein. This approach could accurately differentiate between the target of interest and other bacteria species with enhanced limit of detection of 4.8 × 102CFU/mL. Apple juice artificially contaminated withA. acidoterrestrisat the concentration of 1 CFU/mL could be determined as positive after 24 h enrichment. The resulting immunoassay platform provided a rapid, convenient, sensitive, and selective way for detectingA. acidoterrestris, holding great potential as a robust tool for preventing spoilage in the fruit juice industry.
登录
查看更多内容
用于检测脂环酸杆菌属的多功能荧光磁性纳米粒子的合成。
影响因子：
8.1
作者：
Wang Zhouli;Li Xueke;Zhao Yuejuan;Yuan Yahong;Cai Rui;Yue Tianli
通讯作者：
Yue Tianli
DOI：
--
发表时间：
1998
期刊：
Dairy, food and environmental sanitation
影响因子：
--
作者：
I. Walls;R. Chuyate
通讯作者：
I. Walls;R. Chuyate
DOI：
10.1016/j.trac.2017.08.013
发表时间：
2017-12
期刊：
Trends in Analytical Chemistry
影响因子：
--
作者：
Fanyong Yan;Keqing Fan;Zhangjun Bai;Ruiqi Zhang;Fanlin Zu;Jinxia Xu;Li Xiang
通讯作者：
Fanyong Yan;Keqing Fan;Zhangjun Bai;Ruiqi Zhang;Fanlin Zu;Jinxia Xu;Li Xiang
影响因子：
6.1
作者：
Sophia Mast;R. Dietrich;A. Didier;E. Märtlbauer
通讯作者：
Sophia Mast;R. Dietrich;A. Didier;E. Märtlbauer
影响因子：
6
作者：
Xiao-Chen Huang;Chunfeng Guo;Yahong Yuan;Xin-Xin Luo-Xin;T. Yue
通讯作者：
Xiao-Chen Huang;Chunfeng Guo;Yahong Yuan;Xin-Xin Luo-Xin;T. Yue