The interaction between bone marrow stromal cells and RGD-modified three-dimensional porous polycaprolactone scaffolds.
The interaction between bone marrow stromal cells and RGD-modified three-dimensional porous polycaprolactone scaffolds.
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DOI：
10.1016/j.biomaterials.2009.04.015
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发表时间：
2009-09
期刊：
影响因子：
14
通讯作者：
Hollister, Scott J.
中科院分区：
文献类型：
--
作者：
Zhang, Huina;Lin, Chia-Ying;Hollister, Scott J.
我们之前建立了一种简单的方法，将Arg-Gly-Asp （RGD）肽固定在聚己内酯（PCL）二维膜表面，可显著提高骨髓基质细胞（BMSC）对这些膜的粘附性。目前的工作将这种修饰策略扩展到三维（3D） PCL支架，以研究与未经处理的PCL支架相比，修饰后的PCL支架上BMSCs的附着、细胞分布和细胞结构、信号转导和存活。结果表明，用1,6-己二胺处理三维PCL支架表面，通过茚三醇染色方法检测到，氨基官能团均匀地引入到多孔PCL支架上。通过交联反应，将RGDC肽成功固定在PCL支架表面。虽然本研究中采用的静态播种方法导致细胞分布不均，但RGD修饰的PCL支架仍然表现出改善的BMSC附着和支架内细胞分布。更重要的是，RGD修饰显著上调了整合素介导的信号转导FAK-PI3K-Akt通路，修饰后的支架细胞存活和生长增加。本研究介绍了一种将RGD肽固定在3D多孔PCL支架上的简单方法，并进一步证明了RGD肽修饰3D PCL支架可引起特异性细胞反应，并改善最终的细胞-生物材料相互作用。
We previously established a simple method to immobilize the Arg-Gly-Asp (RGD) peptide on polycaprolactone (PCL) two-dimensional film surfaces that significantly improved bone marrow stromal cell (BMSC) adhesion to these films. The current work extends this modification strategy to three-dimensional (3D) PCL scaffolds to investigate BMSCs attachment, cellular distribution and cellularity, signal transduction and survival on the modified PCL scaffold compared to those on the untreated ones. The results demonstrated that treatment of 3D PCL scaffold surfaces with 1,6-hexanediamine introduced the amino functional groups onto the porous PCL scaffold homogenously as detected by a ninhydrin staining method. Followed by the cross-linking reaction, RGDC peptide was successfully immobilized on the surface of PCL scaffold. Although the static seeding method used in this study caused heterogeneous cell distribution, the RGD modified PCL scaffold still demonstrated the improved BMSC attachment and cellular distribution in the scaffold. More importantly, the integrin-mediated signal transduction FAK-PI3K-Akt pathway was significantly up-regulated by RGD modification and a subsequent increase in cell survival and growth was found in the modified scaffold. The present study introduces an easy method to immobilize RGD peptide on the 3D porous PCL scaffold and provides further evidence that modification of 3D PCL scaffolds with RGD peptides elicits specific cellular responses and improves the final cell-biomaterial interaction.
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