Examination of metabolic responses to phosphorus limitation via proteomic analyses in the marine diatom Phaeodactylum tricornutum.
Examination of metabolic responses to phosphorus limitation via proteomic analyses in the marine diatom Phaeodactylum tricornutum.
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DOI：
10.1038/srep10373
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发表时间：
2015-05-28
影响因子：
4.6
通讯作者：
Li HY
中科院分区：
文献类型：
--
作者：
Feng TY;Yang ZK;Zheng JW;Xie Y;Li DW;Murugan SB;Yang WD;Liu JS;Li HY
磷是海洋浮游植物生存所必需的大量营养元素。本研究从细胞和分子水平研究了三角褐指藻对磷限制的响应。共鉴定出42个非冗余蛋白，其中8个蛋白表达上调，34个蛋白表达下调。结果还表明，与无机磷吸收相关的蛋白质被下调，而参与有机磷吸收的蛋白质如碱性磷酸酶被上调。参与蛋白质降解、脂质积累和光呼吸等代谢反应的蛋白质被上调，而能量代谢、光合作用、氨基酸和核酸代谢则趋于下调。结果表明，三角褐指藻在磷胁迫过程中蛋白质水平发生了变化。本研究为了解磷在海洋生态地球化学循环中的作用以及浮游植物对磷缺乏的响应奠定了基础。研究结果为进一步了解浮游植物群落的演替规律提供了科学依据。
Phosphorus (P) is an essential macronutrient for the survival of marine phytoplankton. In the present study, phytoplankton response to phosphorus limitation was studied by proteomic profiling in diatom Phaeodactylum tricornutum in both cellular and molecular levels. A total of 42 non-redundant proteins were identified, among which 8 proteins were found to be upregulated and 34 proteins were downregulated. The results also showed that the proteins associated with inorganic phosphate uptake were downregulated, whereas the proteins involved in organic phosphorus uptake such as alkaline phosphatase were upregulated. The proteins involved in metabolic responses such as protein degradation, lipid accumulation and photorespiration were upregulated whereas energy metabolism, photosynthesis, amino acid and nucleic acid metabolism tend to be downregulated. Overall our results showed the changes in protein levels of P. tricornutum during phosphorus stress. This study preludes for understanding the role of phosphorous in marine biogeochemical cycles and phytoplankton response to phosphorous scarcity in ocean. It also provides insight into the succession of phytoplankton community, providing scientific basis for elucidating the mechanism of algal blooms.
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