Effects of Aquaporin 4 Knockdown on Brain Edema of the Uninjured Side After Traumatic Brain Injury in Rats.
Effects of Aquaporin 4 Knockdown on Brain Edema of the Uninjured Side After Traumatic Brain Injury in Rats.
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DOI：
10.12659/msm.898190
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发表时间：
2016-12-08
期刊：
影响因子：
--
通讯作者：
Wang W
中科院分区：
文献类型：
--
作者：
Chen JQ;Zhang CC;Jiang SN;Lu H;Wang W
创伤性脑损伤可引起健侧(即对侧脑组织)的水肿。我们评估了AQP4在脑外伤后CBT水肿形成中的作用。采用受控皮质撞击模型建立轻度或重度脑挫伤模型，然后立即脑室注射siRNA。观察AQP4 siRNA对CBT水肿的影响。轻度或重度颅脑损伤可引起不同类型的CBT水肿。此外，与未损伤大鼠(即假手术组)相比，轻度脑损伤后72h脑含水量(BWC)增加，168h后AQP4表达增加。相对于对照siRNA，AQP4干扰在48h后降低了AQP4的表达，72h后降低了BWC的表达。相反，与假手术组相比，重型颅脑损伤后1h BWC增加，1h后AQP4表达一过性增强。然而，与对照siRNA相比，AQP4干扰在1h后降低了AQP4的表达，在24h后降低了BWC的表达。最后，CBT的表观弥散系数(ADC)值与AQP4的表达水平呈正相关。AQP4干扰破坏了这种关联。AQP4干扰可减少CBT水肿的形成，ADC值可预测脑损伤的严重程度。
Traumatic brain injury (TBI) induces edema on the uninjured side (i.e., contralateral brain tissue; CBT). We evaluated the role of AQP4 in CBT edema formation following TBI. Mild or severe TBI was induced using a controlled cortical impact model in rats, immediately followed by intraventricular siRNA infusions. The effects of AQP4 siRNA on CBT edema were assessed at up to 168 h. Mild or severe TBI induced different patterns of CBT edema. Furthermore, following mild TBI, brain water content (BWC) was increased at 72 h thereafter and AQP4 expression was increased after 168 h, relative to non-injured rats (i.e., sham). AQP4 interference reduced AQP4 expression 48 h thereafter and BWC 72 h thereafter, relative to control siRNA. In contrast, following severe TBI, BWC was increased 1 h thereafter and AQP4 expression was transiently enhanced after 1 h, relative to sham. However, AQP4 interference reduced AQP4 expression after 1 h and BWC 24 h thereafter, relative to control siRNA. Finally, apparent diffusion coefficient (ADC) value in CBT was positively correlated with AQP4 expression level following severe, but not mild, TBI. AQP4 interference disrupted this correlation. AQP4 interference reduces CBT edema formation, and ADC value may predict TBI severity.
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