Function and ribosomal localization of aIF6, a translational regulator shared by archaea and eukarya.
Function and ribosomal localization of aIF6, a translational regulator shared by archaea and eukarya.
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DOI：
10.1093/nar/gkn959
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发表时间：
2009-01
影响因子：
14.9
通讯作者：
Londei P
中科院分区：
文献类型：
--
作者：
Benelli D;Marzi S;Mancone C;Alonzi T;la Teana A;Londei P
翻译因子IF 6是真核生物和微生物共有的，但在细菌中没有发现。真核生物IF 6（eIF 6）的特性已经被广泛研究，但仍然有些难以捉摸。eIF 6作为核糖体抗结合因子，参与miRNA介导的基因沉默;然而，它似乎也参与核糖体合成和输出。在这里，我们已经确定了古细菌（硫磺硫化叶菌）IF 6同源物（aIF 6）的功能和核糖体定位。我们发现aIF 6特异性结合50 S核糖体亚基，阻碍70 S核糖体的形成并强烈抑制翻译。aIF 6在细胞周期中沿着均匀表达，但在冷休克和热休克后均上调。aIF 6核糖体结合位点位于50 S亚基的30-S相互作用表面的中间，包括许多参与亚基缔合的关键RNA和蛋白质决定簇。这些数据表明，IF 6蛋白在古真核谱系中进化，在不利的环境条件下调节翻译效率，可能在真核进化过程中获得额外的功能。
The translation factor IF6 is shared by the Archaea and the Eukarya, but is not found in Bacteria. The properties of eukaryal IF6 (eIF6) have been extensively studied, but remain somewhat elusive. eIF6 behaves as a ribosome-anti-association factor and is involved in miRNA-mediated gene silencing; however, it also seems to participate in ribosome synthesis and export. Here we have determined the function and ribosomal localization of the archaeal (Sulfolobus solfataricus) IF6 homologue (aIF6). We find that aIF6 binds specifically to the 50S ribosomal subunits, hindering the formation of 70S ribosomes and strongly inhibiting translation. aIF6 is uniformly expressed along the cell cycle, but it is upregulated following both cold- and heat shock. The aIF6 ribosomal binding site lies in the middle of the 30-S interacting surface of the 50S subunit, including a number of critical RNA and protein determinants involved in subunit association. The data suggest that the IF6 protein evolved in the archaeal–eukaryal lineage to modulate translational efficiency under unfavourable environmental conditions, perhaps acquiring additional functions during eukaryotic evolution.
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影响因子：
64.8
作者：
Gandin, Valentina;Miluzio, Annarita;Barbieri, Anna Maria;Beugnet, Anne;Kiyokawa, Hiroaki;Marchisio, Pier Carlo;Biffo, Stefano
通讯作者：
Biffo, Stefano
DOI：
10.1073/pnas.0607541103
发表时间：
2006-10-24
影响因子：
11.1
作者：
Berk, Veysel;Zhang, Wen;Cate, Jamie H. Doudna
通讯作者：
Cate, Jamie H. Doudna
影响因子：
5.6
作者：
Caserta, Enrico;Tomsic, Jerneja;Gualerzi, Claudio O.
通讯作者：
Gualerzi, Claudio O.
影响因子：
14.9
作者：
Plewniak, F;Bianchetti, L;Poch, O
通讯作者：
Poch, O
影响因子：
5.6
作者：
Graindorge, JS;Rousselle, JC;Fasiolo, F
通讯作者：
Fasiolo, F