Human fetal dermal fibroblast-myeloid cell diversity is characterized by dominance of pro-healing Annexin1-FPR1 signaling.
Human fetal dermal fibroblast-myeloid cell diversity is characterized by dominance of pro-healing Annexin1-FPR1 signaling.
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DOI：
10.1016/j.isci.2023.107533
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发表时间：
2023-09-15
期刊：
影响因子：
5.8
通讯作者：
Sen, Chandan K.
中科院分区：
文献类型：
--
作者：
Srivastava, Rajneesh;Singh, Kanhaiya;Abouhashem, Ahmed S.;Kumar, Manishekhar;Kacar, Sedat;Verma, Sumit S.;Mohanty, Sujit K.;Sinha, Mithun;Ghatak, Subhadip;Xuan, Yi;Sen, Chandan K.
胎儿皮肤实现无疤痕伤口修复。真皮成纤维细胞在细胞外基质沉积和瘢痕形成中起核心作用。胎儿和牙龈伤口修复的结果都是最小的疤痕。我们验证了这样一种假设，即与成人皮肤成纤维细胞相比，人胎儿皮肤成纤维细胞的多样性是独一无二的，并且与牙龈皮肤成纤维细胞部分重叠。在单细胞分辨率下比较了人胎儿皮肤(FS，n=3)、牙龈组织(HGG，n=13)和成熟皮肤(MS，n=13)。真皮成纤维细胞是最丰富的成团细胞，用来建立与其他皮肤细胞的连接体。Annexin1-FPR1信号通路在FS和HGG成纤维细胞及相关髓系细胞中占主导地位，而在MS成纤维细胞中很少。利用组织纳米转染(TNT)技术将髓系特异性FPR1-ORF转移到小鼠伤口边缘，显著提高了愈合质量。假时间分析证实HGG成纤维细胞亚群与胎儿来源的FPR1高髓系细胞共存，表明共同的潜在生物学过程。胎儿皮肤特有的成纤维细胞亚群和牙龈最小化瘢痕胎儿成纤维细胞和髓系细胞共享能量代谢基因集Annexin1-FPR1信号转导促进胎儿皮肤组织修复和再生髓系特异性FPR1通过TNT转导提高伤口愈合质量分子生物学；免疫学；细胞生物学；转录学
Fetal skin achieves scarless wound repair. Dermal fibroblasts play a central role in extracellular matrix deposition and scarring outcomes. Both fetal and gingival wound repair share minimal scarring outcomes. We tested the hypothesis that compared to adult skin fibroblasts, human fetal skin fibroblast diversity is unique and partly overlaps with gingival skin fibroblasts. Human fetal skin (FS, n = 3), gingiva (HGG, n = 13), and mature skin (MS, n = 13) were compared at single-cell resolution. Dermal fibroblasts, the most abundant cluster, were examined to establish a connectome with other skin cells. Annexin1-FPR1 signaling pathway was dominant in both FS as well as HGG fibroblasts and related myeloid cells while scanty in MS fibroblasts. Myeloid-specific FPR1-ORF delivered in murine wound edge using tissue nanotransfection (TNT) technology significantly enhanced the quality of healing. Pseudotime analyses identified the co-existence of an HGG fibroblast subset with FPR1high myeloid cells of fetal origin indicating common underlying biological processes. Fibroblast subpopulation, unique to human fetal skin, and gingiva minimize scar Fetal fibroblasts and myeloid cells share energy metabolism gene sets Annexin1-FPR1 signaling promotes tissue repair and regeneration in fetal skin Myeloid-specific FPR1 delivery via TNT enhances quality of wound healing Molecular biology; Immunology; Cell biology; Transcriptomics
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影响因子：
64.8
作者：
Cao, Junyue;Spielmann, Malte;Shendure, Jay
通讯作者：
Shendure, Jay
DOI：
10.3390/ph14050456
发表时间：
2021-05-12
期刊：
Pharmaceuticals (Basel, Switzerland)
影响因子：
--
作者：
Cho YD;Kim KH;Lee YM;Ku Y;Seol YJ
通讯作者：
Seol YJ
影响因子：
17.1
作者：
Eming SA;Martin P;Tomic-Canic M
通讯作者：
Tomic-Canic M
影响因子：
--
作者：
Jürgensen HJ;Silva LM;Krigslund O;van Putten S;Madsen DH;Behrendt N;Engelholm LH;Bugge TH
通讯作者：
Bugge TH
影响因子：
2.6
作者：
Bullard, KM;Longaker, MT;Lorenz, HP
通讯作者：
Lorenz, HP