Identification of Streptococcus pneumoniae serotype 11E, serovariant 11Av and mixed populations by high-resolution magic angle spinning nuclear magnetic resonance (HR-MAS NMR) spectroscopy and flow cytometric serotyping assay (FCSA).
Identification of Streptococcus pneumoniae serotype 11E, serovariant 11Av and mixed populations by high-resolution magic angle spinning nuclear magnetic resonance (HR-MAS NMR) spectroscopy and flow cytometric serotyping assay (FCSA).
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DOI：
10.1371/journal.pone.0100722
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Pantosti A
中科院分区：
文献类型：
--
作者：
Camilli R;Spencer BL;Moschioni M;Pinto V;Berti F;Nahm MH;Pantosti A
最近的研究已经确定了肺炎链球菌血清型11 E和血清变异11 Av之间的分离株，以前分型为11 A的经典血清分型方法。血清型11 E和血清变体11 Av与血清型11 A的不同之处在于具有完全或部分失活的wcjE，wcjE是cps基因座中编码O-乙酰基转移酶的基因。血清型11 E在携带分离株中很少见，但在侵袭分离株中很常见，这表明它在侵袭过程中存活得更好。这项工作的目的是调查的流行病学血清型11 A的肺炎球菌收集使用一种新的血清分型方法的基础上高分辨率魔角旋转核磁共振（HR-MAS NMR）光谱，以区分血清型11 A和11 E。收集了48株（侵入型34株，携带型14株）沙门氏菌。来自意大利的肺炎分离株（先前通过Quellung反应鉴定为血清型11 A）通过wc 1 E测序、HR-MAS NMR光谱和基于单克隆抗体的参考流式细胞术血清分型测定（FCSA）进行研究。血清型11 A和11 E的HR-MAS NMR谱显示不同的NMR峰，表明HR-MAS NMR可用于区分这些血清型，尽管HR-MAS NMR不能明确区分血清型11 Av和血清型11 E。其中38株为11 A型，8株为11 E型，1株为11 Av型，1株为11 A/11 Av混合群。所有11 E分离株均为侵袭性分离株。我们证明了HR-MAS NMR可以用于肺炎球菌血清分型。在我们收集的侵袭性分离株中检测到血清型11 E，支持了以前的流行病学研究，表明wcjE突变可以代表在侵袭期间促进肺炎球菌存活的机制。在已建立的血清型中发现免疫化学多样性的谱应刺激开发新的血清分型方法的努力。
Recent studies have identified Streptococcus pneumoniae serotype 11E and serovariant 11Av among isolates previously typed as 11A by classical serotyping methods. Serotype 11E and serovariant 11Av differ from serotype 11A by having totally or partially inactive wcjE, a gene in cps locus coding for an O-acetyl transferase. Serotype 11E is rare among carriage isolates but common among invasive isolates suggesting that it survives better during invasion. Aim of this work was to investigate the epidemiology of serotype 11A in a pneumococcal collection using a new serotyping approach based on High-Resolution Magic Angle Spinning Nuclear Magnetic Resonance (HR-MAS NMR) spectroscopy to distinguish serotypes 11A and 11E. A collection of 48 (34 invasive and 14 carriage) S. pneumoniae isolates from Italy, previously identified as serotype 11A by the Quellung reaction, were investigated by wcjE sequencing, HR-MAS NMR spectroscopy and the reference flow cytometric serotyping assay (FCSA) based on monoclonal antibodies. HR-MAS NMR spectra from serotypes 11A and 11E showed different NMR peaks indicating that HR-MAS NMR could be used to distinguish these serotypes, although HR-MAS NMR could not distinguish serotype 11Av from serotype 11E unambiguously. Thirty-eight isolates were confirmed to be serotype 11A, 8 isolates with a mutated wcjE were serotype 11E, 1 isolate belonged to serovariant 11Av, and 1 isolate was a mixed population 11A/11Av. All 11E isolates were identified among invasive isolates. We proved that HR-MAS NMR can be of potential use for pneumococcal serotyping. The detection of serotype 11E among invasive isolates in our collection, supports previous epidemiological studies suggesting that mutations in wcjE can represent a mechanism promoting pneumococcal survival during invasion. The discovery of a spectrum of immunochemical diversity within established serotypes should stimulate efforts to develop new serotyping approaches.
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影响因子：
4.2
作者：
Camilli R;Bonnal RJ;Del Grosso M;Iacono M;Corti G;Rizzi E;Marchetti M;Mulas L;Iannelli F;Superti F;Oggioni MR;De Bellis G;Pantosti A
通讯作者：
Pantosti A
影响因子：
3
作者：
Yu, J.;Carvalho, M. da G. S.;Nahm, M. H.
通讯作者：
Nahm, M. H.
DOI：
10.1097/inf.0b013e31824214ac
发表时间：
2012-03
期刊：
The Pediatric infectious disease journal
影响因子：
--
作者：
Wroe PC;Lee GM;Finkelstein JA;Pelton SI;Hanage WP;Lipsitch M;Stevenson AE;Rifas-Shiman SL;Kleinman K;Dutta-Linn MM;Hinrichsen VL;Lakoma M;Huang SS
通讯作者：
Huang SS
影响因子：
3.7
作者：
Camilli R;Daprai L;Cavrini F;Lombardo D;D'Ambrosio F;Del Grosso M;Vescio MF;Landini MP;Pascucci MG;Torresani E;Garlaschi ML;Sambri V;Pantosti A
通讯作者：
Pantosti A
影响因子：
6.4
作者：
Calix, Juan J.;Nahm, Moon H.
通讯作者：
Nahm, Moon H.