Dissemination of spotted fever rickettsia agents in Europe by migrating birds.
Dissemination of spotted fever rickettsia agents in Europe by migrating birds.
复制标题
通过迁移鸟类来传播欧洲斑点发烧的立克特工。
DOI：
10.1371/journal.pone.0008572
复制
发表时间：
2010-01-05
期刊：
影响因子：
3.7
通讯作者：
Nilsson K
中科院分区：
文献类型：
--
作者：
Elfving K;Olsen B;Bergström S;Waldenström J;Lundkvist A;Sjöstedt A;Mejlon H;Nilsson K
众所周知，候鸟是许多微生物的长距离传播媒介。为了调查立克次体病原体是否也是如此，我们对蜱虫感染进行了表征，并从瑞典 13,260 只候鸟雀形目鸟类中收集了蜱虫。共有 1127 种硬蜱。从这些鸟身上去除蜱虫，并从其中 957 只鸟中提取 DNA 可用于分析。分析 DNA 以检测立克次体属。使用实时 PCR，然后进行 DNA 测序以进行物种鉴定。立克次体属在 108 只（11.3%）蜱虫中检测到微生物。 PCR 阳性样本中主要是瑞士立克次体（一种与人类感染有关的斑疹热立克次体）。在 9 种蜱虫 (0.8%) 中，17kDa 和 ompB 基因的部分序列与立克次体 (Rickettsia monacensis) 和立克次体 sp.IrITA3 株（先前在南欧有过描述，是地中海斑疹热样疾病的病原体）具有最大的相似性。对于 15 种 (1.4%) 蜱虫，17kDa、ompB、gltA 和 ompA 基因与立克次氏体表现出最大的相似性。 Davousti 株、日本立克次体和黑龙江立克次体，均在系统发育上密切相关，前者先前在非洲的 Amblyomma tholloni 蜱中发现，而此前在硬蜱属中未检测到。滴答声。在地面觅食的鸟类中，感染立克次体生物的蜱感染率比其他鸟类高四倍，但这两个群体传播立克次体物种的能力相同。这些鸟似乎不是立克次体属的储存宿主，但在一种情况下，这只鸟似乎患有立克次体病，而且蜱虫从鸟的血液中获得了细菌。总之，迁徙雀形目鸟类是流行病学上重要的媒介蜱和立克次体物种的宿主，并导致斑疹热立克次体病原体及其疾病的地理分布。
Migratory birds are known to play a role as long-distance vectors for many microorganisms. To investigate whether this is true of rickettsial agents as well, we characterized tick infestation and gathered ticks from 13,260 migratory passerine birds in Sweden. A total of 1127 Ixodes spp. ticks were removed from these birds and the extracted DNA from 957 of them was available for analyses. The DNA was assayed for detection of Rickettsia spp. using real-time PCR, followed by DNA sequencing for species identification. Rickettsia spp. organisms were detected in 108 (11.3%) of the ticks. Rickettsia helvetica, a spotted fever rickettsia associated with human infections, was predominant among the PCR-positive samples. In 9 (0.8%) of the ticks, the partial sequences of 17kDa and ompB genes showed the greatest similarity to Rickettsia monacensis, an etiologic agent of Mediterranean spotted fever-like illness, previously described in southern Europe as well as to the Rickettsia sp.IrITA3 strain. For 15 (1.4%) of the ticks, the 17kDa, ompB, gltA and ompA genes showed the greatest similarity to Rickettsia sp. strain Davousti, Rickettsia japonica and Rickettsia heilongjiangensis, all closely phylogenetically related, the former previously found in Amblyomma tholloni ticks in Africa and previously not detected in Ixodes spp. ticks. The infestation prevalence of ticks infected with rickettsial organisms was four times higher among ground foraging birds than among other bird species, but the two groups were equally competent in transmitting Rickettsia species. The birds did not seem to serve as reservoir hosts for Rickettsia spp., but in one case it seems likely that the bird was rickettsiemic and that the ticks had acquired the bacteria from the blood of the bird. In conclusion, migratory passerine birds host epidemiologically important vector ticks and Rickettsia species and contribute to the geographic distribution of spotted fever rickettsial agents and their diseases.
登录
查看更多内容
影响因子：
11.8
作者：
Fournier, PE;Grunnenberger, F;Raoult, D
通讯作者：
Raoult, D
DOI：
10.1099/00207713-47-2-252
发表时间：
1997-04-01
期刊：
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY
影响因子：
--
作者：
Roux, V;Rydkina, E;Raoult, D
通讯作者：
Raoult, D
影响因子：
4.2
作者：
Matsumoto, Kotaro;Parola, Philippe;Raoult, Didier
通讯作者：
Raoult, Didier
影响因子：
2.2
作者：
Santos-Silva, Maria Margarida;Sousa, Rita;Bacellar, Fatima
通讯作者：
Bacellar, Fatima
影响因子：
--
作者：
Palmgren, H;Sellin, M;Olsen, B
通讯作者：
Olsen, B