Focal transcranial magnetic stimulation in awake rats: Enhanced glucose uptake in deep cortical layers.
Focal transcranial magnetic stimulation in awake rats: Enhanced glucose uptake in deep cortical layers.
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DOI：
10.1016/j.jneumeth.2020.108709
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发表时间：
2020-06-01
影响因子：
3
通讯作者：
Lu H
中科院分区：
文献类型：
--
作者：
Cermak S;Meng Q;Peng K;Baldwin S;Mejías-Aponte CA;Yang Y;Lu H
经颅磁刺激（TMS）是一种新兴的神经调节工具。然而，TMS的临床前模型有限。开发在清醒大鼠中进行TMS的方法，并通过绘制TMS给药后的葡萄糖摄取来表征神经元对TMS的反应。在大鼠颅骨内植入一根头柱作为引导TMS靶点的参照物。使用运动阈值测量作为度量来评估跨动物和跨会话的TMS递送的一致性。使用荧光葡萄糖类似物（2-NBDG）作为神经元活动的标记物，我们映射葡萄糖摄取对大鼠运动皮层TMS的响应。平均运动阈值（n=41）为最大刺激输出（MSO）的34.6±6.3%。动物间运动阈值的变异性与人类研究中报告的相似。此外，在3个不同的日子里测量的运动阈值没有显著差异。荧光信号的增强呈剂量（功率）依赖性，以运动皮质为中心，55%MSO时内-外2 mm，头-尾4 mm，35%MSO时3 mm。具有显著荧光信号的总细胞计数为：107±23（55%MSO）、73±11（35%MSO）和42±11（sham，5%MSO）。我们的方法允许一致的运动阈值评估纵向研究。值得注意的是，具有荧光信号增强的细胞一致地聚集在深层皮质层中，在浅层中具有最小的增强。据我们所知，这是第一次在清醒啮齿动物中研究局灶性TMS。
Transcranial magnetic stimulation (TMS) is an emerging neuromodulation tool. However, preclinical models of TMS are limited. To develop a method for performing TMS in awake rats and to characterize neuronal response to TMS by mapping glucose uptake following TMS administration. A headpost was implanted into rat skull serving as a refence to guide TMS target. Motor threshold measurement was used as the metric to assess the consistency in TMS delivery across animals and across sessions. Using a fluorescent glucose analogue (2-NBDG) as a marker of neuronal activity, we mapped glucose uptake in response to TMS of the rat motor cortex. The average motor threshold (n=41) was 34.6±6.3% of maximum stimulator output (MSO). The variability of motor threshold across animals was similar to what has been reported in human studies. Furthermore, there was no significant difference in motor threshold measured across 3 separate days. Enhancement in fluorescent signals were TMS dose (power)-dependent, which centered around the motor cortex, covering an area medial-laterally 2 mm, rostral-caudally 4 mm at 55% MSO, and 3 mm at 35% MSO. The count of total cells with significant fluorescent signal was: 107±23 (55% MSO), 73±11 (35% MSO) and 42±11 (sham, 5% MSO). Our method allows for consistent motor threshold assessment for longitudinal studies. Notably, cells with fluorescent signal enhancement were consistently aggregated in deep cortical layers, with minimal enhancement in superficial layers To our knowledge, this is the first study of focal TMS in awake rodents.
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影响因子：
5.7
作者：
Luber, Bruce M.;Davis, Simon;Strauman, Timothy J.
通讯作者：
Strauman, Timothy J.
影响因子：
82.9
作者：
Drysdale AT;Grosenick L;Downar J;Dunlop K;Mansouri F;Meng Y;Fetcho RN;Zebley B;Oathes DJ;Etkin A;Schatzberg AF;Sudheimer K;Keller J;Mayberg HS;Gunning FM;Alexopoulos GS;Fox MD;Pascual-Leone A;Voss HU;Casey BJ;Dubin MJ;Liston C
通讯作者：
Liston C
重复磁刺激对单皮质锥体神经元的直接影响。
影响因子：
3.7
作者：
Banerjee J;Sorrell ME;Celnik PA;Pelled G
通讯作者：
Pelled G
影响因子：
3.1
作者：
O'Neil, RG;Wu, L;Mullani, N
通讯作者：
Mullani, N
DOI：
10.1016/s0924-980x(97)00036-2
发表时间：
1997-12-01
期刊：
ELECTROMYOGRAPHY AND MOTOR CONTROL-ELECTROENCEPHALOGRAPHY AND CLINICAL NEUROPHYSIOLOGY
影响因子：
--
作者：
Chen, R;Gerloff, C;Cohen, LG
通讯作者：
Cohen, LG