Nucleotide- and Mal3-dependent changes in fission yeast microtubules suggest a structural plasticity view of dynamics.
Nucleotide- and Mal3-dependent changes in fission yeast microtubules suggest a structural plasticity view of dynamics.
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DOI：
10.1038/s41467-017-02241-5
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发表时间：
2017-12-13
影响因子：
16.6
通讯作者：
Moores CA
中科院分区：
文献类型：
--
作者：
von Loeffelholz O;Venables NA;Drummond DR;Katsuki M;Cross R;Moores CA
使用冷冻电子显微镜，我们的特点的微管组装从裂殖酵母微管蛋白，在存在和不存在的情况下，他们的监管合作伙伴Mal 3的架构。冷冻电子断层扫描显示，微管组装从S。粟酒裂殖酵母微管蛋白主要具有B-晶格的原丝间接触，原丝围绕微管轴偏斜。共聚合与Mal 3有利于13原丝微管减少原丝歪斜，表明Mal 3调整interprotofilament接口。微管结合的Mal 3的4.6 μ m分辨率结构表明，Mal 3在S.粟酒裂殖酵母微管晶格与哺乳动物微管不同，S.粟酒裂殖酵母微管不显示与EB蛋白结合和GTP水解相关的纵向晶格压缩。我们的研究结果坚定地支持微管动力学的结构可塑性观点，微管晶格构象对各种效应物敏感，并且对于不同的微管蛋白是不同的。微管是细胞骨架的重要和高度保守的组成部分。在这里，作者进行了结构分析的裂变酵母微管在存在和不存在的微管末端结合蛋白Mal 3，证明微管聚合物之间的结构可塑性。
Using cryo-electron microscopy, we characterize the architecture of microtubules assembled from Schizosaccharomyces pombe tubulin, in the presence and absence of their regulatory partner Mal3. Cryo-electron tomography reveals that microtubules assembled from S. pombe tubulin have predominantly B-lattice interprotofilament contacts, with protofilaments skewed around the microtubule axis. Copolymerization with Mal3 favors 13 protofilament microtubules with reduced protofilament skew, indicating that Mal3 adjusts interprotofilament interfaces. A 4.6-Å resolution structure of microtubule-bound Mal3 shows that Mal3 makes a distinctive footprint on the S. pombe microtubule lattice and that unlike mammalian microtubules, S. pombe microtubules do not show the longitudinal lattice compaction associated with EB protein binding and GTP hydrolysis. Our results firmly support a structural plasticity view of microtubule dynamics in which microtubule lattice conformation is sensitive to a variety of effectors and differently so for different tubulins. Microtubules are vital and highly conserved components of the cytoskeleton. Here the authors carry out a structural analysis of fission yeast microtubules in the presence and absence of the microtubule end-binding protein Mal3 that demonstrates structural plasticity amongst microtubule polymers.
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影响因子：
3.3
作者：
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通讯作者：
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影响因子：
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