Phasic contractions of the mouse vagina and cervix at different phases of the estrus cycle and during late pregnancy.
Phasic contractions of the mouse vagina and cervix at different phases of the estrus cycle and during late pregnancy.
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DOI：
10.1371/journal.pone.0111307
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Jobling P
中科院分区：
文献类型：
--
作者：
Gravina FS;van Helden DF;Kerr KP;de Oliveira RB;Jobling P
激活阴道和宫颈平滑肌相性收缩的起搏机制仍然知之甚少。在这里，我们研究阴道和宫颈组织的起搏特性，确定：1)功能性起搏取决于发情周期或妊娠阶段；2)起搏涉及依赖肌浆/内质网钙-ATPase(SERCA)的细胞内钙释放；以及3)c-Kit和/或波形蛋白免疫活性IC在起搏中起作用。在体外测量阴道和宫颈的收缩，以及c-Kit和波形蛋白阳性间质细胞(ICs)的分布。宫颈平滑肌在动情期和动情期自发活动，而在动情前期和动情间期静止。阴道平滑肌通常是静止的，但在催产素或K+通道阻滞剂四乙基氯化铵(TEA)存在的情况下，表现出阶段性收缩。在低钙溶液或L钙通道阻断剂硝苯地平的存在下，宫颈自发收缩和TEA引起的阴道时相收缩持续存在，但在低钙溶液或硝苯地平的存在下被抑制。在小鼠宫颈中发现少量ICs，而在阴道中未发现ICs。取自动情期、动情期或妊娠晚期小鼠的宫颈肌条一般自发活动。阴道肌条通常是静止的，但可以诱导表现出不同于发情周期或妊娠晚期的阶段性收缩。自发性宫颈或TEA诱导的阴道时相收缩不是由ICs或细胞内钙库介导的。鉴于阴道平滑肌通常是静止的，那么很可能是由于性刺激引起的催产素等激素的增加，从而增强了这种起搏的有效性，直到出现阶段性收缩活动。
The pacemaker mechanisms activating phasic contractions of vaginal and cervical smooth muscle remain poorly understood. Here, we investigate properties of pacemaking in vaginal and cervical tissues by determining whether: 1) functional pacemaking is dependent on the phase of the estrus cycle or pregnancy; 2) pacemaking involves Ca2+ release from sarcoplasmic/endoplasmic reticulum Ca2+-ATPase (SERCA) -dependent intracellular Ca2+ stores; and 3) c-Kit and/or vimentin immunoreactive ICs have a role in pacemaking. Vaginal and cervical contractions were measured in vitro, as was the distribution of c-Kit and vimentin positive interstitial cells (ICs). Cervical smooth muscle was spontaneously active in estrus and metestrus but quiescent during proestrus and diestrus. Vaginal smooth muscle was normally quiescent but exhibited phasic contractions in the presence of oxytocin or the K+ channel blocker tetraethylammonium (TEA) chloride. Spontaneous contractions in the cervix and TEA-induced phasic contractions in the vagina persisted in the presence of cyclopiazonic acid (CPA), a blocker of the SERCA that refills intracellular SR Ca2+ stores, but were inhibited in low Ca2+ solution or in the presence of nifedipine, an inhibitor of L-type Ca2+channels. ICs were found in small numbers in the mouse cervix but not in the vagina. Cervical smooth muscle strips taken from mice in estrus, metestrus or late pregnancy were generally spontaneously active. Vaginal smooth muscle strips were normally quiescent but could be induced to exhibit phasic contractions independent on phase of the estrus cycle or late pregnancy. Spontaneous cervical or TEA-induced vaginal phasic contractions were not mediated by ICs or intracellular Ca2+ stores. Given that vaginal smooth muscle is normally quiescent then it is likely that increases in hormones such as oxytocin, as might occur through sexual stimulation, enhance the effectiveness of such pacemaking until phasic contractile activity emerges.
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影响因子：
3.6
作者：
Duquette, RA;Shmygol, A;Wray, S
通讯作者：
Wray, S
影响因子：
7.3
作者：
Gravina, F. S.;Parkington, H. C.;van Helden, D. F.
通讯作者：
van Helden, D. F.
DOI：
10.1530/jrf.0.0720143
发表时间：
1984-01-01
期刊：
JOURNAL OF REPRODUCTION AND FERTILITY
影响因子：
--
作者：
GARCIAVILLAR, R;TOUTAIN, PL;RUCKEBUSCH, Y
通讯作者：
RUCKEBUSCH, Y
影响因子：
4
作者：
Griffiths, AL;Marshall, KM;Woodward, DF
通讯作者：
Woodward, DF
DOI：
10.1046/j.1439-0442.2003.00583.x
发表时间：
2003-11-01
期刊：
JOURNAL OF VETERINARY MEDICINE SERIES A-PHYSIOLOGY PATHOLOGY CLINICAL MEDICINE
影响因子：
--
作者：
Hirsbrunner, G;Reist, M;Steiner, A
通讯作者：
Steiner, A