Transcriptome Analysis of Calcium- and Hormone-Related Gene Expressions during Different Stages of Peanut Pod Development.
Transcriptome Analysis of Calcium- and Hormone-Related Gene Expressions during Different Stages of Peanut Pod Development.
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花生荚果发育不同阶段钙及激素相关基因表达的转录组分析
DOI：
10.3389/fpls.2017.01241
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发表时间：
2017
影响因子：
5.6
通讯作者：
Li X
中科院分区：
文献类型：
--
作者：
Li Y;Meng J;Yang S;Guo F;Zhang J;Geng Y;Cui L;Wan S;Li X
花生是一种嗜钙植物。钙在大量信号通路中起着无处不在的中心枢纽作用。在田间，缺乏游离钙离子（Ca2+）的土壤会导致荚果不饱满。分析了四个荚果阶段，以确定Ca2+排泄与荚果发育的关系。花生壳在4个阶段均有Ca2+排泄；而第4期胚胎（S4）和第3期红皮（S3）均有Ca2+吸收。结果表明，花生胚和红皮在发育过程中需要Ca2+。为了从基因角度探讨钙、激素与种子发育的关系，我们进一步分析了2期（S2）、S3和S4的种子转录组。产生了大约7000万个高质量的clean reads，这些clean reads被组装成58147个unigenes。通过比较这三个阶段，共鉴定出4,457个差异表达基因。在这些基因中，鉴定出Ca2+相关基因53个，生长素相关基因40个，赤霉素相关基因15个，乙烯相关基因20个，脱落酸相关基因2个，细胞分裂素相关基因7个。并对其中一部分进行了qRT-PCR验证。在豆荚发育过程中，它们的大部分表达发生了变化。由于有报道表明Ca2+信号转导途径参与激素调节途径，这些结果暗示花生种子发育可能是由Ca2+信号转导途径和激素调节途径共同调控的。
Peanut is one of the calciphilous plants. Calcium serves as a ubiquitous central hub in a large number of signaling pathways. In the field, free calcium ion (Ca2+)-deficient soil can result in unfilled pods. Four pod stages were analyzed to determine the relationship between Ca2+ excretion and pod development. Peanut shells showed Ca2+ excretion at all four stages; however, both the embryo of Stage 4 (S4) and the red skin of Stage 3 (S3) showed Ca2+ absorbance. These results showed that embryo and red skin of peanut need Ca2+ during development. In order to survey the relationship among calcium, hormone and seed development from gene perspective, we further analyzed the seed transcriptome at Stage 2 (S2), S3, and S4. About 70 million high quality clean reads were generated, which were assembled into 58,147 unigenes. By comparing these three stages, total 4,457 differentially expressed genes were identified. In these genes, 53 Ca2+ related genes, 40 auxin related genes, 15 gibberellin genes, 20 ethylene related genes, 2 abscisic acid related genes, and 7 cytokinin related genes were identified. Additionally, a part of them were validated by qRT-PCR. Most of their expressions changed during the pod development. Since some reports showed that Ca2+ signal transduction pathway is involved in hormone regulation pathway, these results implied that peanut seed development might be regulated by the collaboration of Ca2+ signal transduction pathway and hormone regulation pathway.
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影响因子：
3.7
作者：
Grandellis C;Fantino E;Muñiz García MN;Bialer MG;Santin F;Capiati DA;Ulloa RM
通讯作者：
Ulloa RM
DOI：
10.3390/molecules20059326
发表时间：
2015-05-21
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Fortes AM;Teixeira RT;Agudelo-Romero P
通讯作者：
Agudelo-Romero P
影响因子：
6.9
作者：
Aharoni, A;O'Connell, AP
通讯作者：
O'Connell, AP
钙依赖性蛋白激酶 CPK28 以蛋氨酸腺苷转移酶为目标，被 26S 蛋白酶体降解，并影响拟南芥中的乙烯生物合成和木质素沉积
影响因子：
7.2
作者：
Jin, Yu;Ye, Nenghui;Zhang, Jianhua
通讯作者：
Zhang, Jianhua
影响因子：
7.4
作者：
Cannon, SB;Crow, JA;Retzel, EF
通讯作者：
Retzel, EF