Mice lacking FABP9/PERF15 develop sperm head abnormalities but are fertile.
Mice lacking FABP9/PERF15 develop sperm head abnormalities but are fertile.
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DOI：
10.1016/j.ydbio.2010.09.019
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发表时间：
2010-12-15
影响因子：
2.7
通讯作者：
Travis AJ
中科院分区：
文献类型：
--
作者：
Selvaraj V;Asano A;Page JL;Nelson JL;Kothapalli KS;Foster JA;Brenna JT;Weiss RS;Travis AJ
The male germ cell-specific fatty acid binding protein 9 (FABP9/PERF15) is the major component of the murine sperm perforatorium and perinuclear theca. Based on its cytoskeletal association and sequence homology to myelin P2 (FABP8), it has been suggested that FABP9 tethers sperm membranes to the underlying cytoskeleton. Furthermore, its upregulation in apoptotic testicular germ cells and its increased phosphorylation status during capacitation suggested multiple important functions for FABP9. Therefore, we investigated specific functions for FABP9 by means of targeted gene disruption in mice. FABP9−/− mice were viable and fertile. Phenotypic analysis showed that FABP9−/− mice had significant increases in sperm head abnormalities (~8% greater than their WT cohorts); in particular, we observed the reduction or absence of the characteristic structural element known as the “ventral spur” in ~10% of FABP9−/− sperm. However, deficiency of FABP9 neither affected membrane tethering to the perinuclear theca nor the fatty acid composition of sperm. Moreover, epididymal sperm numbers were not affected in FABP9−/− mice. Therefore, we conclude that FABP9 plays only a minor role in providing the murine sperm head its characteristic shape and is not absolutely required for spermatogenesis or sperm function.
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影响因子：
14.9
作者：
Eswar, N;John, B;Sali, A
通讯作者：
Sali, A
DOI：
10.1107/s0907444905014162
发表时间：
2005-08-01
影响因子：
2.2
作者：
Hunter, DJB;Macmaster, R;Freer, AA
通讯作者：
Freer, AA
DOI：
10.1095/biolreprod2.supplement_2.90
发表时间：
1970-01-01
期刊：
Biol. Reprod.
影响因子：
--
作者：
Fawcett, D. W.
通讯作者：
Fawcett, D. W.
影响因子：
5.6
作者：
Asano, Atsushi;Selvaraj, Vimal;Travis, Alexander J.
通讯作者：
Travis, Alexander J.
DOI：
10.1071/bi9510581
发表时间：
1951-01-01
期刊：
AUSTRALIAN JOURNAL OF SCIENTIFIC RESEARCH SERIES B-BIOLOGICAL SCIENCES
影响因子：
--
作者：
AUSTIN, CR
通讯作者：
AUSTIN, CR