Bacteriophage t4 nanoparticles as materials in sensor applications: variables that influence their organization and assembly on surfaces.

Bacteriophage t4 nanoparticles as materials in sensor applications: variables that influence their organization and assembly on surfaces.
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DOI:
10.3390/s90806298
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发表时间:
2009
期刊:
Sensors (Basel, Switzerland)
影响因子:
--
通讯作者:
Liu JL
Liu JL
中科院分区:
其他
文献类型:
--
作者:
Archer MJ;Liu JL

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噬菌体 T4 纳米颗粒所具有的特性使其成为传感器材料(特别是传感器探针)的理想候选材料。它们的表面可以通过基因工程或直接化学缀合进行修饰,以显示功能部分,例如抗体或其他蛋白质,以识别特定目标。然而,为了将 T4 纳米颗粒用作传感器探针,有必要了解和控制决定其在表面上组装和组织的变量。这项工作的目的是讨论我们确定的影响 T4 纳米粒子在表面行为的一些变量。使用原子力显微镜 (AFM) 定性研究 pH、离子强度、基质特性、纳米颗粒浓度和电荷的影响。
Bacteriophage T4 nanoparticles possess characteristics that make them ideal candidates as materials for sensors, particularly as sensor probes. Their surface can be modified, either through genetic engineering or direct chemical conjugation to display functional moieties such as antibodies or other proteins to recognize a specific target. However, in order for T4 nanoparticles to be utilized as a sensor probe, it is necessary to understand and control the variables that determine their assembly and organization on a surface. The aim of this work is to discuss some of variables that we have identified as influencing the behavior of T4 nanoparticles on surfaces. The effect of pH, ionic strength, substrate characteristics, nanoparticle concentration and charge was addressed qualitatively using atomic force microscopy (AFM).
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