The Effects of Interstitial Lung Diseases on Alveolar Extracellular Vesicles Profile: A Multicenter Study.
The Effects of Interstitial Lung Diseases on Alveolar Extracellular Vesicles Profile: A Multicenter Study.
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DOI：
10.3390/ijms24044071
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发表时间：
2023-02-17
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
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作者：
间质性肺疾病（ILD）的诊断是困难的。细胞外囊泡（EVs）促进细胞间的通讯，并由多种细胞释放。我们的目的是研究特发性肺纤维化（IPF）、结节病和过敏性肺炎（HP）患者支气管肺泡灌洗（BAL）中的EV标志物。纳入了在锡耶纳、巴塞罗那和福贾大学医院随访的ILD患者。BAL上清液用于分离ev。通过MACSPlex Exsome KIT进行流式细胞术鉴定。肺泡内大部分EV标记物与纤维化损伤有关。IPF患者肺泡标本中仅表达CD56、CD105、CD142、CD31和CD49e，而HP仅表达CD86和CD24。一些EV标记物（CD11c、CD1c、CD209、CD4、CD40、CD44、CD8）在HP和结节病之间是共同的。主成分分析基于EV标记区分了三组，总方差为60.08%。本研究证明了流式细胞术方法在BAL样品中表型和表征EV表面标记物的有效性。两种肉芽肿性疾病，结节病和HP，在IPF患者中没有发现肺泡EV标志物。我们的研究结果证明了肺泡室的生存能力，可以识别肺特异性的IPF和HP标志物。
Diagnosis of interstitial lung diseases (ILD) is difficult to perform. Extracellular vesicles (EVs) facilitate cell-to-cell communication, and they are released by a variety of cells. Our goal aimed to investigate EV markers in bronchoalveolar lavage (BAL) from idiopathic pulmonary fibrosis (IPF), sarcoidosis and hypersensitivity pneumonitis (HP) cohorts. ILD patients followed at Siena, Barcelona and Foggia University Hospitals were enrolled. BAL supernatants were used to isolate the EVs. They were characterized by flow cytometry assay through MACSPlex Exsome KIT. The majority of alveolar EV markers were related to the fibrotic damage. CD56, CD105, CD142, CD31 and CD49e were exclusively expressed by alveolar samples from IPF patients, while HP showed only CD86 and CD24. Some EV markers were common between HP and sarcoidosis (CD11c, CD1c, CD209, CD4, CD40, CD44, CD8). Principal component analysis distinguished the three groups based on EV markers with total variance of 60.08%. This study has demonstrated the validity of the flow cytometric method to phenotype and characterize EV surface markers in BAL samples. The two granulomatous diseases, sarcoidosis and HP, cohorts shared alveolar EV markers not revealed in IPF patients. Our findings demonstrated the viability of the alveolar compartment allowing identification of lung-specific markers for IPF and HP.
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DOI：
10.3390/life11121401
发表时间：
2021-12-15
期刊：
Life (Basel, Switzerland)
影响因子：
--
作者：
d'Alessandro M;Bergantini L;Bargagli E;Vidal S
通讯作者：
Vidal S
影响因子：
5.6
作者：
Kendall RT;Feghali-Bostwick CA
通讯作者：
Feghali-Bostwick CA
影响因子：
9.6
作者：
Hanumegowda, Chandru;Farkas, Laszlo;Kolb, Martin
通讯作者：
Kolb, Martin
DOI：
10.1164/rccm.202008-3093oc
发表时间：
2021-07-15
影响因子：
24.7
作者：
Huang, Yong;Oldham, Justin M.;Noth, Imre
通讯作者：
Noth, Imre
影响因子：
6.1
作者：
d'Alessandro, Miriana;Bergantini, Laura;Bargagi, Elena
通讯作者：
Bargagi, Elena