Myofibrillogenesis in skeletal muscle cells in zebrafish.
Myofibrillogenesis in skeletal muscle cells in zebrafish.
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DOI：
10.1002/cm.20365
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发表时间：
2009-08
影响因子：
--
通讯作者：
Sanger, Jean M.
中科院分区：
文献类型：
--
作者：
Sanger, Joseph W.;Wang, Jushuo;Holloway, Beth;Du, Aiping;Sanger, Jean M.
关键词：
基于在培养的禽类肌肉细胞中的观察，肌原纤维发生的“前肌原纤维”模型提出，成熟的肌原纤维之前有两个中间结构：前肌原纤维和新生肌原纤维。为了确定该模型是否适用于斑马鱼骨骼肌发育，我们用肌节α-辅肌动蛋白和肌球蛋白II的抗体对发育中的胚胎进行染色。在最年轻的肌肉细胞中，肌节α-辅肌动蛋白和非肌肉肌球蛋白II分别位于类似于禽肌细胞中的前肌原纤维的小条带的线性阵列中。肌肉特异性肌球蛋白II的分布开始作为分散的短纤维，随后在时间上由重叠的纤维束和有组织的A-带在老年体节。α-辅肌动蛋白组织从小的Z-体珠状Z-带和肌原纤维中的Z-带，延长了伸长体节的长度。在较老的体节与成熟的肌原纤维，premyofibrils也存在于成熟的肌原纤维的末端，这表明随着细胞和体节的增长更长，premyofibrils参与现有的成熟肌原纤维的伸长。光漂白后的荧光恢复表明，蛋白质（肌动蛋白，α-辅肌动蛋白，脂肪酸，肌球蛋白和telethonin）之间的肌浆和Z-带的成熟肌原纤维在斑马鱼类似于相同的蛋白质在培养的禽肌管中看到的交换，这表明肌原纤维组装和维护在斑马鱼与禽肌肉有共同的属性。
The “premyofibril” model of myofibrillogenesis, based on observations in cultured avian muscle cells, proposes that mature myofibrils are preceded by two intermediary structures: premyofibrils and nascent myofibrils. To determine if this model applies to zebrafish skeletal muscle development, we stained developing embryos with antibodies to sarcomeric alpha-actinin and myosin II. In the youngest muscle cells, sarcomeric alpha-actinin and non-muscle myosin II were each localized in linear arrays of small bands that resembled the premyofibrils in avian myocytes. The distribution of muscle–specific myosin II began as scattered short filaments followed in time by overlapping bundles of filaments and organized A-bands in the older somites. Alpha-actinin organization changed from small z-bodies to beaded Z-bands and ordered Z-bands in myofibrils that extended the length of the elongating somites. In older somites with mature myofibrils, premyofibrils were also present at the ends of the mature myofibrils, suggesting that as the cells and somites grew longer, premyofibrils were involved in the elongation of existing mature myofibrils. Fluorescence Recovery After Photobleaching showed that the exchange of proteins (actin, alpha-actinin, FATZ, myotilin and telethonin) between sarcoplasm and the Z-bands of mature myofibrils in zebrafish resembled that seen for the same proteins in cultured avian myotubes, suggesting that myofibril assembly and maintenance in zebrafish share common properties with avian muscle.
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影响因子：
2.7
作者：
FELSENFELD, AL;CURRY, M;KIMMEL, CB
通讯作者：
KIMMEL, CB
影响因子：
1.5
作者：
Holtzer, H;Hijikata, T;Sweeney, HL
通讯作者：
Sweeney, HL
影响因子：
2.5
作者：
KIMMEL, CB;BALLARD, WW;SCHILLING, TF
通讯作者：
SCHILLING, TF
DOI：
10.1073/pnas.94.17.9493
发表时间：
1997-08-19
影响因子：
11.1
作者：
Dabiri, GA;Turnacioglu, KK;Sanger, JW
通讯作者：
Sanger, JW
影响因子：
2.7
作者：
Du, Aiping;Sanger, Jean M.;Sanger, Joseph W.
通讯作者：
Sanger, Joseph W.