Skeletal Muscle Cell Growth Alters the Lipid Composition of Extracellular Vesicles.
Skeletal Muscle Cell Growth Alters the Lipid Composition of Extracellular Vesicles.
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DOI：
10.3390/membranes11080619
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发表时间：
2021-08-12
期刊：
影响因子：
4.2
通讯作者：
Vechetti IJ
中科院分区：
文献类型：
--
作者：
Valentino TR;Rule BD;Mobley CB;Nikolova-Karakashian M;Vechetti IJ
我们试图表征骨骼肌细胞来源的细胞外小泡(EVS)的脂类分布，以确定肥大刺激是否会影响C2C12肌管来源EVS的脂质成分。分析包括将C2C12小鼠成肌细胞分化为肌管，并用胰岛素样生长因子1(IGF-1)处理24小时以诱导肥大生长。从细胞培养上清液中分离EVS，用纳米颗粒跟踪分析(NTA)进行定量，并用透射电子显微镜(TEM)进行分析。将EVS匀浆，提取脂类，用质谱仪进行定量，然后进行下游脂类的浓缩和脂链分析。IGF-1治疗组CD63和CD81水平(39%和21%)分别高于对照组(16%)。分析表明，骨骼肌源性EV富含生物活性脂类，在肥大生长过程中可能选择性地并入EV中。IGF-1处理肌管对分泌的EV中的二酰甘油(DG)和神经酰胺(Cer)水平有显著影响。具体地说，与对照细胞相比，来自IGF处理细胞的EV中不饱和DG的比例高出两到三倍。饱和DG水平未受影响。在C16-和C24-Cer中也出现了类似的选择性增加，但在其他物种中没有。游离狮身人面肌的碱基水平有下降的趋势，而鞘氨醇-1-磷酸的水平不受影响。我们的结果表明，骨骼肌源性EVS的脂质组成和生物发生在肥大生长过程中具有特异性和高度的选择性。
We sought to characterize the lipid profile of skeletal muscle cell-derived Extracellular Vesicles (EVs) to determine if a hypertrophic stimulus would affect the lipid composition of C2C12 myotube-derived EVs. Analyses included C2C12 murine myoblasts differentiated into myotubes and treated with Insulin-Like Growth Factor 1 (IGF-1) for 24 h to induce hypertrophic growth. EVs were isolated from cell culture media, quantified using Nanoparticle Tracking Analysis (NTA) and analyzed using Transmission Electron Microscopy (TEM). EVs were homogenized and lipids extracted for quantification by Mass Spectrometry followed by downstream lipid class enrichment and lipid chain analysis. IGF-1 treatment elicited an increase in CD63 and CD81 levels (39% and 21%) compared to the controls (16%), respectively. Analysis revealed that skeletal muscle-derived EVs are enriched in bioactive lipids that are likely selectively incorporated into EVs during hypertrophic growth. IGF-1 treatment of myotubes had a significant impact on the levels of diacylglycerol (DG) and ceramide (Cer) in secreted EVs. Specifically, the proportion of unsaturated DG was two- to three-fold higher in EVs derived from IGF-treated cells, as compared to those from control cells. The levels of saturated DG were unaffected. Selective increases were similarly seen in C16- and C24-Cer but not in other species. Levels of free sphingoid bases tended to decrease, while those of sphingosine-1-phosphate was unaffected. Our results suggest that the lipid composition and biogenesis of skeletal muscle-derived EVs, are specific and highly selective during hypertrophic growth.
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影响因子：
3.7
作者：
Duong, Phat;Chung, Allen;Raffai, Robert L.
通讯作者：
Raffai, Robert L.
DOI：
10.1083/jcb.200508014
发表时间：
2006-03-13
期刊：
The Journal of cell biology
影响因子：
--
作者：
Booth AM;Fang Y;Fallon JK;Yang JM;Hildreth JE;Gould SJ
通讯作者：
Gould SJ
影响因子：
4.6
作者：
Cui S;Sun B;Yin X;Guo X;Chao D;Zhang C;Zhang CY;Chen X;Ma J
通讯作者：
Ma J
影响因子：
82.9
作者：
Erion DM;Shulman GI
通讯作者：
Shulman GI
影响因子：
4.8
作者：
Alonso, R;Rodríguez, MC;Izquierdo, M
通讯作者：
Izquierdo, M