Carbamylated albumin is one of the target antigens of anti-carbamylated protein antibodies.
Carbamylated albumin is one of the target antigens of anti-carbamylated protein antibodies.
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DOI：
10.1093/rheumatology/kex088
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发表时间：
2017-07-01
期刊：
影响因子：
--
通讯作者：
Ohmura K
中科院分区：
文献类型：
--
作者：
Nakabo S;Hashimoto M;Ito S;Furu M;Ito H;Fujii T;Yoshifuji H;Imura Y;Nakashima R;Murakami K;Kuramoto N;Tanaka M;Satoh J;Ishigami A;Morita S;Mimori T;Ohmura K
目标。在 RA 患者中检测到抗氨甲酰化蛋白（抗 CarP）抗体。抗CarP ELISA采用胎牛血清作为抗原源，目前尚未找到精确的靶抗原。我们的目的是鉴定抗 CarP 抗体的靶抗原。 方法。进行抗CarP抗体的蛋白质印迹。通过内部 ELISA 检测 493 名 RA 患者和 144 名健康对照 (HC) 的抗氨甲酰化人白蛋白 (CarALB) 抗体。使用 8 名 RA 患者的血清进行了 CarALB 和瓜氨酸白蛋白 (citALB) 的抗 CarP 抗体抑制 ELISA。采用液相色谱-串联质谱法（LC/MS/MS）检测血清CarALB，采用ELISA法测定血清MPO浓度。 结果。我们关注氨甲酰化白蛋白，因为它对应于抗 CarP 抗体蛋白质印迹检测到的最厚条带的大小。 31.4% 的 RA 患者检测到抗 CarALB 抗体，抗 CarALB 和抗 CarP 之间的滴度相关性比抗 citALB 和抗 CCP 抗体之间的滴度相关性更接近（分别为 ρ = 0.59 和 ρ = 0.16）。抑制 ELISA 显示抗 CarP 抗体被 CarALB 抑制，但不被 citALB 抑制。通过 LC/MS/MS 在 RA 患者的血清中检测到 CarALB。血清 MPO 浓度与疾病活动度相关，并且具有抗 CarALB 抗体的 RA 患者的血清 MPO 浓度高于没有抗 CarALB 抗体的患者。 结论。我们发现氨甲酰化白蛋白是抗CarP抗体的一种新型靶抗原，也是第一个报道但尚未报道为ACPA靶点的靶抗原。
Objectives. Anti-carbamylated protein (anti-CarP) antibodies are detected in RA patients. Fetal calf serum is used as an antigen source in anti-CarP ELISA, and the precise target antigens have not been found. We aimed to identify the target antigens of anti-CarP antibodies. Methods. Western blotting of anti-CarP antibodies was conducted. Anti-carbamylated human albumin (CarALB) antibody was detected by in-house ELISA for 493 RA patients and 144 healthy controls (HCs). An inhibition ELISA of anti-CarP antibodies by CarALB and citrullinated albumin (citALB) was performed using eight RA patients’ sera. Serum CarALB was detected by liquid chromatography–tandem mass spectroscopy (LC/MS/MS), and the serum MPO concentration was measured by ELISA. Results. We focused on carbamylated albumin because it corresponded to the size of the thickest band detected by western blotting of anti-CarP antibodies. Anti-CarALB antibody was detected in 31.4% of RA patients, and the correlation of the titres between anti-CarALB and anti-CarP was much closer than that between anti-citALB and anti-CCP antibodies (ρ = 0.59 and ρ = 0.16, respectively). The inhibition ELISA showed that anti-CarP antibodies were inhibited by CarALB, but not by citALB. CarALB was detected in sera from RA patients by LC/MS/MS. The serum MPO concentration was correlated with disease activity and was higher in RA patients with anti-CarALB antibody than in those without. Conclusion. We found that carbamylated albumin is a novel target antigen of anti-CarP antibodies, and it is the first reported target antigen that has not been reported as the target of ACPA.
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DOI：
10.4049/jimmunol.1000075
发表时间：
2010-06-15
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Mydel P;Wang Z;Brisslert M;Hellvard A;Dahlberg LE;Hazen SL;Bokarewa M
通讯作者：
Bokarewa M
DOI：
10.1007/bf00405277
发表时间：
1987-01-01
影响因子：
3
作者：
HUSGAFVELPURSIAINEN, K;SORSA, M;EINISTO, P
通讯作者：
EINISTO, P
影响因子：
4.9
作者：
Di Giuseppe D;Discacciati A;Orsini N;Wolk A
通讯作者：
Wolk A
影响因子：
5.5
作者：
Meune, Christophe;Touze, Emmanuel;Allanore, Yannick
通讯作者：
Allanore, Yannick
影响因子：
4
作者：
Martínez G;Gómez JA;Bang H;Martínez-Gamboa L;Roggenbuck D;Burmester GR;Torres B;Prada D;Feist E
通讯作者：
Feist E