Macrophages Are Involved in Gut Bacterial Translocation and Reversed by Lactobacillus in Experimental Uremia
Macrophages Are Involved in Gut Bacterial Translocation and Reversed by Lactobacillus in Experimental Uremia
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实验性尿毒症中巨噬细胞参与肠道细菌易位并被乳酸菌逆转
DOI：
10.1007/s10620-015-3950-z
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发表时间：
2016-06
影响因子：
3.1
通讯作者：
何荃
中科院分区：
文献类型：
--
作者：
孙凌霜;刘华;蒋红利;魏萌;梁珊珊;王萌;史珂慧;何荃
背景：尿毒症引起肠道微生物群失调，其特征是有益细菌减少。肠道细菌易位(BT)导致尿毒症的微炎症，这与不良后果有关。巨噬细胞是否参与Bt的发生尚不清楚。目的：探讨巨噬细胞参与Bt和尿毒症微炎症及乳酸杆菌Lb对巨噬细胞活性的影响。方法：雄性SD大鼠随机分为假手术组、尿毒症组和尿毒症+益生菌组。荧光显微镜下观察肠道和肠外组织中的巨噬细胞和GFP标记的示踪菌。用透射电子显微镜观察巨噬细胞的超微结构。免疫组织化学方法检测分化簇11a(CD11a)、诱导型一氧化氮合酶(INOS)和细胞间黏附分子-1(ICAM-1)的表达。RT-PCR和Western印迹检测早期生长反应基因1(Egr1)和Toll样受体4(TLR4)的mRNA和蛋白表达。结果：尿毒症大鼠肠道和肠外组织中可见GFP标记的示踪细菌和巨噬细胞共存。与假手术组相比，尿毒症大鼠巨噬细胞胞浆突起和伪足明显减少。乳酸菌LB液治疗后可恢复假足和突起。与假手术组相比，尿毒症组大鼠肠道巨噬细胞CD11a、iNOS、ICAM-1表达增强，TLR4、EGR1表达增强。结论：尿毒症大鼠肠道巨噬细胞偏向促炎表型，导致微炎症。吞噬功能受损的巨噬细胞与BT有关。乳杆菌Lb通过增强巨噬细胞吞噬功能来降低Bt。
Background:Uremia causes gut microbiome dysbiosis, which is characterized by a reduction in beneficial bacteria. Intestinal bacterial translocation (BT) contributes to microinflammation in uremia, which is associated with adverse outcomes. Whether macrophages are involved in BT remains unclear.Aims:We investigated the involvement of macrophages in BT and microinflammation in uremic rats and whether Lactobacillus LB can influence macrophage activity.Methods:Male Sprague-Dawley rats were randomly divided into three groups: sham, uremia, and uremia + probiotic. Macrophages and GFP-labeled tracer bacteria in intestinal and extraintestinal tissues were observed by fluorescence microscopy. The macrophage ultrastructure was examined by transmission electron microscopy. Immunochemistry was used to analyze the expression of cluster of differentiation 11a (CD11a), inducible nitric oxide synthase (iNOS), and intercellular adhesion molecule-1 (ICAM-1). RT-PCR and Western blot were employed to assess the mRNA and protein expression of early growth response gene 1 (EGR1) and toll-like receptor 4 (TLR4).Results:In uremic rats, the colocalization of GFP-labeled tracer bacteria and macrophages was visible in intestinal and extraintestinal tissues. Compared with the sham group, the uremic macrophages showed fewer cytoplasmic protrusions and pseudopodia. Administration of Lactobacillus LB restored the protrusions and pseudopodia. Compared with the sham group, the uremia group exhibited macrophages with higher staining intensities for CD11a, iNOS, and ICAM-1, and higher mRNA and protein expression of TLR4 and EGR1.Conclusions:Intestinal macrophages in the uremic rats are polarized toward a proinflammatory phenotype, resulting in microinflammation. Macrophages with impaired phagocytic function are associated with BT. Lactobacillus LB reduces BT by enhancing macrophage phagocytosis.
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影响因子：
3.1
作者：
V. Delcenserie;D. Martel;M. Lamoureux;J. Amiot;Y. Boutin;Debarati Roy
通讯作者：
V. Delcenserie;D. Martel;M. Lamoureux;J. Amiot;Y. Boutin;Debarati Roy
保加利亚乳杆菌突变体分解尿毒症毒素
影响因子：
3
作者：
Jiang Yun-Sheng;Bai Yun-Huan;Jiang Ya-Fen
通讯作者：
Jiang Ya-Fen
影响因子：
3.1
作者：
Wang, Feiqian;Zhang, Pan;Cheng, Shaoli
通讯作者：
Cheng, Shaoli
影响因子：
4.4
作者：
Xu, ZJ;Dziarski, R;Gupta, D
通讯作者：
Gupta, D
影响因子：
4.6
作者：
Mafra D;Fouque D
通讯作者：
Fouque D