Microfluidic Platform for the Long-Term On-Chip Cultivation of Mammalian Cells for Lab-On-A-Chip Applications.
Microfluidic Platform for the Long-Term On-Chip Cultivation of Mammalian Cells for Lab-On-A-Chip Applications.
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DOI：
10.3390/s17071603
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发表时间：
2017-07-10
期刊：
影响因子：
--
通讯作者：
Vellekoop MJ
中科院分区：
文献类型：
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作者：
Bunge F;Driesche SVD;Vellekoop MJ
由于缺乏方便的细胞培养设备，用于哺乳动物细胞长期分析的芯片实验室（LoC）应用仍然非常罕见。困难在于整合合适的供应结构，需要昂贵的设备，如孵化器和复杂的泵，以及材料的选择。本器械由坚硬但无细胞毒性的材料（硅和玻璃）制成，包含两个垂直排列的水凝胶膜。多孔膜用于将培养室与用于气体和营养物的两个供应通道分开。细胞通过扩散穿过膜而连续进料，而不需要培养箱，并且对向组件供应培养基的要求低。模拟氧气的扩散，以找到腔室的最佳尺寸。芯片通过3D打印支架连接到宏观世界。支架涂有Parlyene C，以确保仅生物相容性材料与培养基接触。MDCK细胞的实验表明，成功地接种在芯片内，培养和传代。因此，所提出的平台是迈向需要长期培养哺乳动物细胞的芯片实验室应用的一步。
Lab-on-a-Chip (LoC) applications for the long-term analysis of mammalian cells are still very rare due to the lack of convenient cell cultivation devices. The difficulties are the integration of suitable supply structures, the need of expensive equipment like an incubator and sophisticated pumps as well as the choice of material. The presented device is made out of hard, but non-cytotoxic materials (silicon and glass) and contains two vertical arranged membranes out of hydrogel. The porous membranes are used to separate the culture chamber from two supply channels for gases and nutrients. The cells are fed continuously by diffusion through the membranes without the need of an incubator and low requirements on the supply of medium to the assembly. The diffusion of oxygen is modelled in order to find the optimal dimensions of the chamber. The chip is connected via 3D-printed holders to the macroscopic world. The holders are coated with Parlyene C to ensure that only biocompatible materials are in contact with the culture medium. The experiments with MDCK-cells show the successful seeding inside the chip, culturing and passaging. Consequently, the presented platform is a step towards Lab-on-a-Chip applications that require long-term cultivation of mammalian cells.
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DOI：
10.3390/s150820030
发表时间：
2015-08-14
期刊：
Sensors (Basel, Switzerland)
影响因子：
--
作者：
Grist SM;Schmok JC;Liu MC;Chrostowski L;Cheung KC
通讯作者：
Cheung KC
影响因子：
2.9
作者：
Sidorenko, Yury;Wahl, Aljoscha;Reichl, Udo
通讯作者：
Reichl, Udo
影响因子：
3.2
作者：
Acosta, Miguel A.;Jiang, Xiao;Gamcsik, Michael P.
通讯作者：
Gamcsik, Michael P.
影响因子：
3.9
作者：
Li, ZY;Chang, SC;Williams, RS
通讯作者：
Williams, RS
影响因子：
4.6
作者：
Lei KF;Tseng HP;Lee CY;Tsang NM
通讯作者：
Tsang NM