Immune Responses in Pregnant Sows Induced by Recombinant Lactobacillus johnsonii Expressing the COE Protein of Porcine Epidemic Diarrhea Virus Provide Protection for Piglets against PEDV Infection.
Immune Responses in Pregnant Sows Induced by Recombinant Lactobacillus johnsonii Expressing the COE Protein of Porcine Epidemic Diarrhea Virus Provide Protection for Piglets against PEDV Infection.
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表达猪流行性腹泻病毒 COE 蛋白的重组约氏乳杆菌诱导怀孕母猪的免疫反应为仔猪提供抗 PEDV 感染的保护
DOI：
10.3390/v14010007
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发表时间：
2021-12-21
期刊：
影响因子：
--
通讯作者：
Tang L
中科院分区：
文献类型：
--
作者：
Zheng D;Wang X;Ju N;Wang Z;Sui L;Wang L;Qiao X;Cui W;Jiang Y;Zhou H;Li Y;Tang L
猪流行性腹泻(PED)是由猪流行性腹泻病毒(PEDV)引起的猪肠道传染病，给养猪业造成严重的经济损失。为了开发针对 PEDV 感染的有效口服疫苗，我们使用猪源约氏乳杆菌 (L. johnsonii) 作为抗原递送载体。重组菌株pPG-T7g10-COE/L。产生了表达 COE 蛋白（病毒刺突蛋白的中和表位）的约氏乳杆菌（L. johnsonii-COE）。口服给药后评价对体外树突状细胞的免疫调节作用和妊娠母猪的免疫原性。约氏乳杆菌-COE 可以激活单核细胞源性树突状细胞 (MoDC) 成熟并引发细胞免疫反应。口服约氏乳杆菌-COE 疫苗后，怀孕母猪体内的抗 PEDV 特异性血清 IgG、IgA 和 IgM 抗体以及粘膜分泌型免疫球蛋白 A (SIgA) 抗体水平均被诱导。母乳中检测到高水平的PEDV特异性SIgA和IgG抗体，这为仔猪抵抗PEDV感染提供了有效的保护。总之，口服约氏乳杆菌-COE 能够有效激活抗 PEDV 体液和细胞免疫反应，显示出作为母猪疫苗的潜力，为仔猪提供针对 PEDV 的保护。
Porcine epidemic diarrhea (PED) induced by porcine epidemic diarrhea virus (PEDV) is an intestinal infectious disease in pigs that causes serious economic losses to the pig industry. To develop an effective oral vaccine against PEDV infection, we used a swine-origin Lactobacillus johnsonii (L. johnsonii) as an antigen delivery carrier. A recombinant strain pPG-T7g10-COE/L. johnsonii (L. johnsonii-COE) expressing COE protein (a neutralizing epitope of the viral spike protein) was generated. The immunomodulatory effect on dendritic cell in vitro and immunogenicity in pregnant sows was evaluated following oral administration. L. johnsonii-COE could activate monocyte-derived dendritic cell (MoDC) maturation and triggered cell immune responses. After oral vaccination with L. johnsonii-COE, levels of anti-PEDV-specific serum IgG, IgA, and IgM antibodies as well as mucosal secretory immunoglobulin A (SIgA) antibody were induced in pregnant sows. High levels of PEDV-specific SIgA and IgG antibodies were detected in the maternal milk, which provide effective protection for the piglets against PEDV infection. In summary, oral L. johnsonii-COE was able to efficiently activate anti-PEDV humoral and cellular immune responses, demonstrating potential as a vaccine for use in sows to provide protection of their piglets against PEDV.
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影响因子：
2.4
作者：
Song DS;Oh JS;Kang BK;Yang JS;Moon HJ;Yoo HS;Jang YS;Park BK
通讯作者：
Park BK
影响因子：
7.3
作者：
Ma, Sunting;Qiao, Xinyuan;Li, Yijing
通讯作者：
Li, Yijing
影响因子：
5
作者：
Song, Liying;Xie, Weichun;Tang, Lijie
通讯作者：
Tang, Lijie
DOI：
10.1038/nri2335
发表时间：
2008-06
期刊：
Nature reviews. Immunology
影响因子：
--
作者：
通讯作者：
--
影响因子：
6.4
作者：
Summerfield, A;Guzylack-Piriou, L;McCullough, KC
通讯作者：
McCullough, KC