Oral efficacy of Vero cell attenuated porcine epidemic diarrhea virus DR13 strain.
Oral efficacy of Vero cell attenuated porcine epidemic diarrhea virus DR13 strain.
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DOI：
10.1016/j.rvsc.2006.03.007
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发表时间：
2007-03
影响因子：
2.4
通讯作者：
Park BK
中科院分区：
文献类型：
--
作者：
Song DS;Oh JS;Kang BK;Yang JS;Moon HJ;Yoo HS;Jang YS;Park BK
应用限制性内切酶片段长度多态性（RFLP）技术对猪流行性腹泻病毒（PEDV）Vero细胞弱毒株DR 13与野生型PEDV进行了鉴别。将细胞减毒的DR 13口服或肌内（IM）施用给晚期妊娠母猪，并在两个不同组的被动免疫的哺乳仔猪中研究由高毒力PEDV攻击导致的死亡率。口服组的死亡率（13%）低于IM组（60%）。特别地，与IM组相比，口服组中母猪的仔猪中抗PEDV的伊加浓度更高。减毒的DR 13病毒仍然是安全的，即使在仔猪中三次传代后。本研究的发现支持了以下理论：Vero细胞减毒DR 13病毒可用作口服疫苗，用于在晚期妊娠母猪中诱导特异性免疫，具有针对PEDV感染的高保护范围。
A Vero cell attenuated porcine epidemic diarrhea virus (PEDV) strain, DR13, was distinguished from wild-type PEDV using restriction enzyme fragment length polymorphism (RFLP). Cell attenuated DR13 was orally or intramuscularly (IM) administered to late-term pregnant sows, and mortality resulting from the highly virulent PEDV challenge was investigated in passively immunized suckling piglets of the two different groups. The mortality rate of the oral group (13%) was lower than that of the IM group (60%). In particular, the concentration of IgA against PEDV was higher in piglets of sows in the oral group, compared to the IM group. The attenuated DR13 virus remained safe, even after three backpassages in piglets. The findings of this study support the theory that the Vero cell attenuated DR13 virus may be applied as an oral vaccine for inducing specific immunity in late-term pregnant sows with a high margin of protection against PEDV infection.
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