Substrate-dependent assembly of the Tat translocase as observed in live Escherichia coli cells.
Substrate-dependent assembly of the Tat translocase as observed in live Escherichia coli cells.
复制标题
DOI：
10.1371/journal.pone.0069488
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Müller M
中科院分区：
文献类型：
--
作者：
Rose P;Fröbel J;Graumann PL;Müller M
The twin-arginine translocation (Tat) pathway guides fully folded proteins across membranes of bacteria, archaea and plant chloroplasts. In Escherichia coli, Tat-specific transport is executed in a still largely unknown manner by three functionally diverse membrane proteins, termed TatA, TatB, and TatC. In order to follow the intracellular distribution of the TatABC proteins in live E. coli cells, we have individually expressed fluorophore-tagged versions of each Tat protein in addition to a set of chromosomally encoded TatABC proteins. In this way, a Tat translocase could form from the native TatABC proteins and be visualized via the association of a fluorescent Tat variant. A functionally active TatA-green fluorescent protein fusion was found to re-locate from a uniform distribution in the membrane into a few clusters preferentially located at the cell poles. Clustering was absolutely dependent on the co-expression of functional Tat substrates, the proton-motive force, and the cognate TatBC subunits. Likewise, polar cluster formation of a functional TatB-mCherry fusion required TatA and TatC and that of a functional TatC-mCherry fusion a functional Tat substrate. Furthermore we directly demonstrate the co-localization of TatA and TatB in the same fluorescent clusters. Our collective results are consistent with distinct Tat translocation sites dynamically forming in vivo in response to newly synthesized Tat substrates.
登录
查看更多内容
DOI：
10.1073/pnas.76.9.4530
发表时间：
1979-01-01
影响因子：
11.1
作者：
CASADABAN, MJ;COHEN, SN
通讯作者：
COHEN, SN
影响因子：
2.9
作者：
Chan, Catherine S.;Zlomislic, Marian R.;Turner, Raymond J.
通讯作者：
Turner, Raymond J.
依赖于Tha4依赖性转运之前的类囊体三个依赖性前体蛋白与CPTATC-HCF106复合物结合。
影响因子：
7.8
作者：
Cline, K;Mori, H
通讯作者：
Mori, H
影响因子：
4.8
作者：
Dabney-Smith, C;Mori, H;Cline, K
通讯作者：
Cline, K
影响因子：
3.5
作者：
Berthelmann, F;Brüser, T
通讯作者：
Brüser, T