Temperature Driven Changes in Benthic Bacterial Diversity Influences Biogeochemical Cycling in Coastal Sediments.
Temperature Driven Changes in Benthic Bacterial Diversity Influences Biogeochemical Cycling in Coastal Sediments.
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温度驱动的底栖细菌多样性变化影响沿海沉积物的生物地球化学循环。
DOI：
10.3389/fmicb.2018.01730
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发表时间：
2018
影响因子：
5.2
通讯作者：
Duncan KR
中科院分区：
文献类型：
--
作者：
Hicks N;Liu X;Gregory R;Kenny J;Lucaci A;Lenzi L;Paterson DM;Duncan KR
海洋沉积物是由大型动物和微藻介导的全球生物地球化学循环的重要场所。然而，驱动这些关键过程的是微生物。有强有力的证据表明，沿海底栖栖息地将受到环境变量变化（气温升高、二氧化碳浓度升高）的影响，研究通常集中在对大型动物生物多样性和生态系统服务的影响上。尽管微生物群落很重要，但人们对微生物群落如何响应环境变化的了解还较少。在这项研究中，采用了操纵性中生态实验，利用下一代测序来评估未来环境变化情景下微生物群落的变化。 Illumina 测序生成了超过 1100 万个 16S rRNA 基因序列（使用偏向于细菌的引物组），并揭示了拟杆菌门和变形菌门在沉积物样品的总细菌群落中占主导地位。在这项研究中，测序覆盖度和深度揭示了一些门内物种丰度的明显变化。细菌群落组成与模拟环境条件相关，物种水平群落组成受环境平均温度的显着影响（p = 0.002），但不受二氧化碳变化或昼夜温度变化的影响。物种水平随平均温度升高的变化与 NH4 浓度的变化相对应，表明微生物群落中氮循环不存在功能冗余。未来环境条件下的海洋沿海生物地球化学循环可能是由微生物活动直接导致的养分可用性变化驱动的。
Marine sediments are important sites for global biogeochemical cycling, mediated by macrofauna and microalgae. However, it is the microorganisms that drive these key processes. There is strong evidence that coastal benthic habitats will be affected by changing environmental variables (rising temperature, elevated CO2), and research has generally focused on the impact on macrofaunal biodiversity and ecosystem services. Despite their importance, there is less understanding of how microbial community assemblages will respond to environmental changes. In this study, a manipulative mesocosm experiment was employed, using next-generation sequencing to assess changes in microbial communities under future environmental change scenarios. Illumina sequencing generated over 11 million 16S rRNA gene sequences (using a primer set biased toward bacteria) and revealed Bacteroidetes and Proteobacteria dominated the total bacterial community of sediment samples. In this study, the sequencing coverage and depth revealed clear changes in species abundance within some phyla. Bacterial community composition was correlated with simulated environmental conditions, and species level community composition was significantly influenced by the mean temperature of the environmental regime (p = 0.002), but not by variation in CO2 or diurnal temperature variation. Species level changes with increasing mean temperature corresponded with changes in NH4 concentration, suggesting there is no functional redundancy in microbial communities for nitrogen cycling. Marine coastal biogeochemical cycling under future environmental conditions is likely to be driven by changes in nutrient availability as a direct result of microbial activity.
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影响因子：
5.2
作者：
Comte J;Fauteux L;Del Giorgio PA
通讯作者：
Del Giorgio PA
海洋微生物基因的丰度和社区组成，以响应海洋酸化和温度升高，这两个对比沿海海洋沉积物。
影响因子：
5.2
作者：
Currie AR;Tait K;Parry H;de Francisco-Mora B;Hicks N;Osborn AM;Widdicombe S;Stahl H
通讯作者：
Stahl H
影响因子：
2.1
作者：
Albuquerque, L;Simoes, C;da Costa, MS
通讯作者：
da Costa, MS
影响因子：
3.7
作者：
CHAO, A;LEE, SM
通讯作者：
LEE, SM
DOI：
10.1073/pnas.1000080107
发表时间：
2011-03-15
影响因子：
11.1
作者：
Caporaso, J. Gregory;Lauber, Christian L.;Knight, Rob
通讯作者：
Knight, Rob