Neuronal mechanisms of learning and memory revealed by spatial and temporal suppression of neurotransmission using shibire, a temperature-sensitive dynamin mutant gene in Drosophila melanogaster.
Neuronal mechanisms of learning and memory revealed by spatial and temporal suppression of neurotransmission using shibire, a temperature-sensitive dynamin mutant gene in Drosophila melanogaster.
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DOI：
10.3389/neuro.02.011.2009
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发表时间：
2009
影响因子：
4.8
通讯作者：
Kitamoto T
中科院分区：
文献类型：
--
作者：
Kasuya J;Ishimoto H;Kitamoto T
果蝇Drosophila melanogaster是一种很好的模式生物，可以识别对学习和记忆很重要的基因和遗传途径。然而，它的小尺寸使得手术治疗和电生理操作在技术上很困难，阻碍了在记忆处理中起关键作用的神经元回路的功能分析。为了解决这个问题，我们开发了一种独特的实验策略，该策略使用果蝇动力蛋白基因的温度敏感等位基因shibirets 1（shits 1）与GAL 4/UAS表达系统相结合。这种策略允许在可识别的神经元中快速和可逆地扰动突触神经传递，并分析这种操纵在自由移动动物中的行为后果。自2001年引入以来，GAL 4/UAS-shits 1策略已被广泛用于研究学习和记忆的神经元基础。这篇综述主要介绍了这一策略如何振兴果蝇记忆研究，并有助于我们了解控制记忆各个方面的动态神经元过程。
The fruit fly Drosophila melanogaster is an excellent model organism to identify genes and genetic pathways important for learning and memory. However, its small size makes surgical treatment and electrophysiological manipulation technically difficult, hampering the functional analysis of neuronal circuits that play critical roles in memory processing. To circumvent this problem, we developed a unique experimental strategy that uses the temperature-sensitive allele of the Drosophila dynamin gene, shibirets1 (shits1), in combination with the GAL4/UAS expression system. This strategy allows for rapid and reversible perturbation of synaptic neurotransmission in identifiable neurons, and analysis of the behavioral consequences of such manipulation in free-moving animals. Since its introduction in 2001, this GAL4/UAS-shits1 strategy has been widely used to study the neuronal basis of learning and memory. This review focuses on how this strategy has revitalized Drosophila memory research, and contributed to our understanding of dynamic neuronal processes that control various aspects of memory.
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影响因子：
9.2
作者：
Riemensperger, T;Völler, T;Fiala, A
通讯作者：
Fiala, A
影响因子：
1.9
作者：
HEISENBERG, M;BORST, A;BYERS, D
通讯作者：
BYERS, D
DOI：
10.1002/neu.480140305
发表时间：
1983-01-01
期刊：
JOURNAL OF NEUROBIOLOGY
影响因子：
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作者：
KOSAKA, T;IKEDA, K
通讯作者：
IKEDA, K
影响因子：
56.9
作者：
Isabel, G;Pascual, A;Preat, T
通讯作者：
Preat, T
影响因子：
9.2
作者：
Schroll, Christian;Riemensperger, Thomas;Fiala, Andre
通讯作者：
Fiala, Andre