Characterization of the Bat proteins in the oxidative stress response of Leptospira biflexa.
Characterization of the Bat proteins in the oxidative stress response of Leptospira biflexa.
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DOI：
10.1186/1471-2180-12-290
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发表时间：
2012-12-13
期刊：
影响因子：
4.2
通讯作者：
Rosa PA
中科院分区：
文献类型：
--
作者：
Stewart PE;Carroll JA;Dorward DW;Stone HH;Sarkar A;Picardeau M;Rosa PA
钩端螺旋体缺乏其他细菌中存在的许多氧化防御同源物，但确实编码拟杆菌耐氧（Bat）蛋白的同源物，这些蛋白已被认为可以实现这一功能。螺旋体门的所有科中均已鉴定出蝙蝠同源物，但这些蛋白质的特定功能尚未得到实验证明。我们研究了模式生物双折钩端螺旋体中蝙蝠蛋白的贡献，了解它们对生长速率、形态和抵御氧化挑战的潜在贡献。与野生型菌株相比，所有蝙蝠 ORF 均被删除的基因工程突变菌株并未表现出生长速度或形态的改变。我们也无法证明蝙蝠蛋白在应对各种氧化应激方面的保护作用。此外，与其他细菌物种中所显示的情况相反，将双折乳杆菌预先暴露于亚致死水平的活性氧似乎不会诱导一般的氧化应激反应。对野生型和突变株的差异蛋白质组分析仅检测到单一蛋白质 HtpG 丰度的变化，该蛋白质由蝙蝠基因座下游的基因编码。这里提供的数据并不支持之前提出的钩端螺旋体蝙蝠蛋白在直接应对氧化应激方面的保护作用。 L. biflexa 对超氧化物和 H2O2 等活性氧相对敏感，表明这种螺旋体尽管是严格需氧菌，但缺乏针对氧化损伤的强大保护性防御。
Leptospires lack many of the homologs for oxidative defense present in other bacteria, but do encode homologs of the Bacteriodes aerotolerance (Bat) proteins, which have been proposed to fulfill this function. Bat homologs have been identified in all families of the phylum Spirochaetes, yet a specific function for these proteins has not been experimentally demonstrated. We investigated the contribution of the Bat proteins in the model organism Leptospira biflexa for their potential contributions to growth rate, morphology and protection against oxidative challenges. A genetically engineered mutant strain in which all bat ORFs were deleted did not exhibit altered growth rate or morphology, relative to the wild-type strain. Nor could we demonstrate a protective role for the Bat proteins in coping with various oxidative stresses. Further, pre-exposing L. biflexa to sublethal levels of reactive oxygen species did not appear to induce a general oxidative stress response, in contrast to what has been shown in other bacterial species. Differential proteomic analysis of the wild-type and mutant strains detected changes in the abundance of a single protein only – HtpG, which is encoded by the gene immediately downstream of the bat loci. The data presented here do not support a protective role for the Leptospira Bat proteins in directly coping with oxidative stress as previously proposed. L. biflexa is relatively sensitive to reactive oxygen species such as superoxide and H2O2, suggesting that this spirochete lacks a strong, protective defense against oxidative damage despite being a strict aerobe.
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影响因子：
3.1
作者：
Lo, Miranda;Murray, Gerald L.;Adler, Ben
通讯作者：
Adler, Ben
影响因子：
3.2
作者：
JOHNSON, RC;HARRIS, VG
通讯作者：
HARRIS, VG
影响因子：
3.2
作者：
IMLAY, JA;LINN, S
通讯作者：
LINN, S
影响因子：
64.8
作者：
DEMPLE, B;HALBROOK, J
通讯作者：
HALBROOK, J
影响因子：
64.5
作者：
LEE, JO;RIEU, P;LIDDINGTON, R
通讯作者：
LIDDINGTON, R