Bioinformatic characterisation of the effector repertoire of the strawberry pathogen Phytophthora cactorum
Bioinformatic characterisation of the effector repertoire of the strawberry pathogen Phytophthora cactorum
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草莓病原菌疫霉效应子库的生物信息学特征
DOI:
10.1101/321141
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发表时间:
2018
期刊:
影响因子:
--
通讯作者:
Armitage A
中科院分区:
文献类型:
--
作者:
Armitage A
The oomycete pathogenPhytophthora cactorumcauses crown rot, a major disease of cultivated strawberry. We report the draft genome ofP.cactorumisolate 10300, isolated from symptomaticFragariaxananassatissue. Our analysis revealed that there are a large number of genes encoding putative secreted effectors in the genome, including nearly 200 RxLR domain containing effectors, 77 Crinklers (CRN) grouped into 38 families, and numerous apoplastic effectors, such as phytotoxins (PcF proteins) and necrosis inducing proteins. As in otherPhytophthoraspecies, the genomic environment of many RxLR and CRN genes differed from core eukaryotic genes, a hallmark of the two-speed genome. We found genes homologous to knownPhytophthora infestansavirulence genes includingAvr1,Avr3b,Avr4,Avrblb1andAvrSmira2indicating effector sequence conservation betweenPhytophthoraspecies of clade 1a and clade 1c. The reportedP.cactorumgenome sequence and associated annotations represent a comprehensive resource for avirulence gene discovery in other Phytophthora species from clade 1 and, will facilitate effector informed breeding strategies in other crops.
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