Structural basis of transcription activation by the global regulator Spx.
Structural basis of transcription activation by the global regulator Spx.
复制标题
全局调节因子 Spx 转录激活的结构基础。
DOI：
10.1093/nar/gkab790
复制
发表时间：
2021-10-11
影响因子：
14.9
通讯作者：
Lin W
中科院分区：
文献类型：
--
作者：
Shi J;Li F;Wen A;Yu L;Wang L;Wang F;Jin Y;Jin S;Feng Y;Lin W
Spx is a global transcriptional regulator in Gram-positive bacteria and has been inferred to efficiently activate transcription upon oxidative stress by engaging RNA polymerase (RNAP) and promoter DNA. However, the precise mechanism by which it interacts with RNAP and promoter DNA to initiate transcription remains obscure. Here, we report the cryo-EM structure of an intact Spx-dependent transcription activation complex (Spx–TAC) from Bacillus subtilis at 4.2 Å resolution. The structure traps Spx in an active conformation and defines key interactions accounting for Spx-dependent transcription activation. Strikingly, an oxidized Spx monomer engages RNAP by simultaneously interacting with the C-terminal domain of RNAP alpha subunit (αCTD) and σA. The interface between Spx and αCTD is distinct from those previously reported activators, indicating αCTD as a multiple target for the interaction between RNAP and various transcription activators. Notably, Spx specifically wraps the conserved –44 element of promoter DNA, thereby stabilizing Spx–TAC. Besides, Spx interacts extensively with σA through three different interfaces and promotes Spx-dependent transcription activation. Together, our structural and biochemical results provide a novel mechanistic framework for the regulation of bacterial transcription activation and shed new light on the physiological roles of the global Spx-family transcription factors.
登录
查看更多内容
影响因子：
5.7
作者：
Awad, Wael;Al-Eryani, Yusra;von Wachenfeldt, Claes
通讯作者：
von Wachenfeldt, Claes
影响因子：
64.5
作者：
Feklistov A;Darst SA
通讯作者：
Darst SA
DOI：
10.1107/s0907444904019158
发表时间：
2004-12-01
影响因子：
2.2
作者：
Emsley, P;Cowtan, K
通讯作者：
Cowtan, K
影响因子：
10.5
作者：
Lloyd, GS;Niu, W;Busby, SJW
通讯作者：
Busby, SJW
DOI：
10.1006/bbrc.2002.6559
发表时间：
2002-03-08
影响因子：
3.1
作者：
Griffith, KL;Shah, IM;Wolf, RE
通讯作者：
Wolf, RE