Therapeutic effects of human amniotic fluid-derived stem cells on renal interstitial fibrosis in a murine model of unilateral ureteral obstruction.
Therapeutic effects of human amniotic fluid-derived stem cells on renal interstitial fibrosis in a murine model of unilateral ureteral obstruction.
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DOI：
10.1371/journal.pone.0065042
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Xiao A
中科院分区：
文献类型：
--
作者：
Sun D;Bu L;Liu C;Yin Z;Zhou X;Li X;Xiao A
间质纤维化被认为是慢性肾脏病（CKD）进展的主要途径，通常与严重的肾功能不全相关。基于干细胞的疗法可能为CKD的治疗提供替代方法。人羊水干细胞（human amniotic fluid derived stem cells，hAFSCs）是一类具有胚胎干细胞和间充质干细胞特性的新型干细胞。在此，本研究调查了在单侧输尿管梗阻（UUO）的小鼠模型中，将hAFSC移植到肾组织中是否可以改善肾间质纤维化。我们发现，hAFSC提供了保护作用，并减轻了间质纤维化，如微血管密度增加所反映的;此外，hAFSC治疗有益地调节了血管内皮生长因子（VEGF）、缺氧诱导因子-1 α（HIF-1α）和转化生长因子-β1（TGF-β1）的蛋白水平。因此，我们假设hAFSC可以代表可用于治疗肾间质纤维化的替代的、容易获得的干细胞来源。
Interstitial fibrosis is regarded as the main pathway for the progression of chronic kidney disease (CKD) and is often associated with severe renal dysfunction. Stem cell-based therapies may provide alternative approaches for the treatment of CKD. Human amniotic fluid-derived stem cells (hAFSCs) are a novel stem cell population, which exhibit both embryonic and mesenchymal stem cell characteristics. Herein, the present study investigated whether the transplantation of hAFSCs into renal tissues could improve renal interstitial fibrosis in a murine model of unilateral ureteral obstruction (UUO). We showed that hAFSCs provided a protective effect and alleviated interstitial fibrosis as reflected by an increase in microvascular density; additionally, hAFSCs treatment beneficially modulated protein levels of vascular endothelial growth factor (VEGF), hypoxia inducible factor-1α (HIF-1α) and transforming growth factor-β1 (TGF-β1). Therefore, we hypothesize that hAFSCs could represent an alternative, readily available source of stem cells that can be applied for the treatment of renal interstitial fibrosis.
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