Baculovirus infection induces disruption of the nuclear lamina.
Baculovirus infection induces disruption of the nuclear lamina.
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杆状病毒感染引起核纤层破坏
DOI：
10.1038/s41598-017-08437-5
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发表时间：
2017-08-10
影响因子：
4.6
通讯作者：
Yuan M
中科院分区：
文献类型：
--
作者：
Zhang X;Xu K;Wei D;Wu W;Yang K;Yuan M
杆状病毒核衣壳主要通过在核膜上出芽而从核中排出。核膜下面的核纤层代表了核出口的实质性屏障。核纤层是否在杆状病毒感染过程中受到破坏仍不清楚。在这份报告中，我们产生了一个克隆细胞系，Sf 9-L，稳定表达GFP标记的果蝇核纤层蛋白B。GFP自发荧光与免疫荧光抗核纤层蛋白B共定位于Sf 9-L细胞的核边缘，表明GFP-核纤层蛋白B掺入核纤层中。病毒在Sf 9-L细胞中能正常增殖。接下来，我们研究了杆状病毒感染Sf 9-L细胞期间核纤层的改变。部分GFP-核纤层蛋白B弥散分布于核边缘，部分GFP-核纤层蛋白B在感染晚期重新分布于核内，表明核纤层部分被破坏。免疫电镜显示GFP-核纤层蛋白B与病毒发生间质的电子致密间质基质、核内微泡、ODV包膜和核内核衣壳的边缘之间存在关联，表明核纤层释放了一些GFP-核纤层蛋白B。此外，感染后GFP-核纤层蛋白B磷酸化增加。基于这些数据，杆状病毒感染诱导核纤层蛋白B磷酸化和破坏。
Baculovirus nucleocapsids egress from the nucleus primarily via budding at the nuclear membrane. The nuclear lamina underlying the nuclear membrane represents a substantial barrier to nuclear egress. Whether the nuclear lamina undergoes disruption during baculovirus infection remains unknown. In this report, we generated a clonal cell line, Sf9-L, that stably expresses GFP-tagged Drosophila lamin B. GFP autofluorescence colocalized with immunofluorescent anti-lamin B at the nuclear rim of Sf9-L cells, indicating GFP-lamin B was incorporated into the nuclear lamina. Meanwhile, virus was able to replicate normally in Sf9-L cells. Next, we investigated alterations to the nuclear lamina during baculovirus infection in Sf9-L cells. A portion of GFP-lamin B localized diffusely at the nuclear rim, and some GFP-lamin B was redistributed within the nucleus during the late phase of infection, suggesting the nuclear lamina was partially disrupted. Immunoelectron microscopy revealed associations between GFP-lamin B and the edges of the electron-dense stromal mattes of the virogenic stroma, intranuclear microvesicles, and ODV envelopes and nucleocapsids within the nucleus, indicating the release of some GFP-lamin B from the nuclear lamina. Additionally, GFP-lamin B phosphorylation increased upon infection. Based on these data, baculovirus infection induced lamin B phosphorylation and disruption of the nuclear lamina.
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比较蛋白质组学揭示杆状病毒两个子代表型之间的基本结构和功能差异
影响因子：
5.4
作者：
Hou, Dianhai;Zhang, Leike;Hu, Zhihong
通讯作者：
Hu, Zhihong
影响因子：
3.9
作者：
Goldberg, Martin W.;Fiserova, Jindriska;Stick, Reimer
通讯作者：
Stick, Reimer
影响因子：
5.4
作者：
Chen, Yun-Ru;Zhong, Silin;Blissard, Gary W.
通讯作者：
Blissard, Gary W.
DOI：
10.1073/pnas.0402727101
发表时间：
2004-06-01
影响因子：
11.1
作者：
Braunagel, SC;Williamson, ST;Summers, MD
通讯作者：
Summers, MD
影响因子：
7
作者：
Kinoshita, E;Kinoshita-Kikuta, E;Koike, T
通讯作者：
Koike, T