NOP132 is required for proper nucleolus localization of DEAD-box RNA helicase DDX47.
NOP132 is required for proper nucleolus localization of DEAD-box RNA helicase DDX47.
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DOI：
10.1093/nar/gkl603
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发表时间：
2006
影响因子：
14.9
通讯作者：
Nishimoto T
中科院分区：
文献类型：
--
作者：
Sekiguchi T;Hayano T;Yanagida M;Takahashi N;Nishimoto T
以前，我们描述了一种新的核仁蛋白，NOP 132，它与小GTP结合蛋白RRAGA相互作用。为了阐明NOP 132在核仁中的功能，我们使用质谱方法鉴定了与NOP 132相互作用的蛋白质。NOP 132主要与参与核糖体生物发生和RNA代谢的蛋白质相关，包括DEAD盒RNA解旋酶蛋白DDX 47，其酵母同系物是Rrp3，其在前rRNA加工中起作用。FLAG标记的DDX47共沉淀rRNA前体的免疫沉淀，以及可能参与核糖体生物合成的许多蛋白质，这意味着DDX47在前rRNA加工中发挥作用。NOP132小干扰RNA的引入诱导了DDX47在核仁中的环状定位，表明NOP132是DDX47在核仁内适当定位所必需的。我们提出，NOP 132的功能在招聘的前rRNA加工蛋白，包括DDX 47，到该地区的rRNA是在核仁内转录。
Previously, we described a novel nucleolar protein, NOP132, which interacts with the small GTP binding protein RRAG A. To elucidate the function of NOP132 in the nucleolus, we identified proteins that interact with NOP132 using mass spectrometric methods. NOP132 associated mainly with proteins involved in ribosome biogenesis and RNA metabolism, including the DEAD-box RNA helicase protein, DDX47, whose yeast homolog is Rrp3, which has roles in pre-rRNA processing. Immunoprecipitation of FLAG-tagged DDX47 co-precipitated rRNA precursors, as well as a number of proteins that are probably involved in ribosome biogenesis, implying that DDX47 plays a role in pre-rRNA processing. Introduction of NOP132 small interfering RNAs induced a ring-like localization of DDX47 in the nucleolus, suggesting that NOP132 is required for the appropriate localization of DDX47 within the nucleolus. We propose that NOP132 functions in the recruitment of pre-rRNA processing proteins, including DDX47, to the region where rRNA is transcribed within the nucleolus.
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