A quantitative study of neurochemically defined populations of inhibitory interneurons in the superficial dorsal horn of the mouse spinal cord.
A quantitative study of neurochemically defined populations of inhibitory interneurons in the superficial dorsal horn of the mouse spinal cord.
复制标题
DOI：
10.1016/j.neuroscience.2017.08.044
复制
发表时间：
2017-11-05
期刊：
影响因子：
3.3
通讯作者：
Todd AJ
中科院分区：
文献类型：
--
作者：
Boyle KA;Gutierrez-Mecinas M;Polgár E;Mooney N;O'Connor E;Furuta T;Watanabe M;Todd AJ
小鼠I-II层抑制神经元的神经化学与大鼠相似。5种神经化学类可解释小鼠所有的I-II层抑制神经元。兴奋性运动啡细胞主要局限于无毛皮肤区域。背角1 - 2层约四分之一的神经元为抑制性中间神经元。这些在调节体感信息中起着重要作用，包括被感知为疼痛或瘙痒的信息。先前在大鼠身上的研究发现，这些细胞中有四种基本不重叠的神经化学物质群，通过丙氨酸、神经肽Y （NPY）、神经元一氧化氮合酶（nNOS）或小蛋白的表达来定义。随后发现丙氨酸细胞共表达肌啡肽。最近的几项研究使用转基因小鼠来研究不同中间神经元群体的功能，因此确定相同的模式是否适用于小鼠，并估计这些群体的相对大小是很重要的。我们发现小鼠浅表背角抑制性中间神经元的神经化学组织与大鼠相似，尽管这些神经元表达NPY的比例更大（33%）。在它们之间，这四个种群占I-II层中抑制细胞的75%。由于该区域约25%的抑制性中间神经元属于一种新的calcalins表达类型，我们的研究结果表明，几乎所有浅表背角的抑制性中间神经元都可以被分配到这五种神经化学群体中的一种。虽然我们主要关注的是抑制性神经元，但我们也在I-II层中发现了一群表达兴奋性运动啡的细胞，这些细胞主要局限于腰椎中背角的内侧，与无毛皮肤区域相对应。这些发现对于利用分子遗传学技术操纵中间神经元群体的功能来研究它们在体感觉加工中的作用的研究具有重要意义。
Neurochemistry of lamina I–II inhibitory neurons in mouse is similar to that in rat. Five neurochemical classes account for all lamina I–II inhibitory neurons in mouse. Excitatory dynorphin cells are largely restricted to glabrous skin territory. Around a quarter of neurons in laminae I–II of the dorsal horn are inhibitory interneurons. These play an important role in modulating somatosensory information, including that perceived as pain or itch. Previous studies in rat identified four largely non-overlapping neurochemical populations among these cells, defined by expression of galanin, neuropeptide Y (NPY), neuronal nitric oxide synthase (nNOS) or parvalbumin. The galanin cells were subsequently shown to coexpress dynorphin. Several recent studies have used genetically modified mice to investigate the function of different interneuron populations, and it is therefore important to determine whether the same pattern applies in mouse, and to estimate the relative sizes of these populations. We show that the neurochemical organization of inhibitory interneurons in mouse superficial dorsal horn is similar to that in the rat, although a larger proportion of these neurons (33%) express NPY. Between them, these four populations account for ∼75% of inhibitory cells in laminae I–II. Since ∼25% of inhibitory interneurons in this region belong to a novel calretinin-expressing type, our results suggest that virtually all inhibitory interneurons in superficial dorsal horn can be assigned to one of these five neurochemical populations. Although our main focus was inhibitory neurons, we also identified a population of excitatory dynorphin-expressing cells in laminae I–II that are largely restricted to the medial part of the mid-lumbar dorsal horn, corresponding to glabrous skin territory. These findings are important for interpretation of studies using molecular-genetic techniques to manipulate the functions of interneuron populations to investigate their roles in somatosensory processing.
登录
查看更多内容
影响因子：
7.4
作者：
Cameron D;Polgár E;Gutierrez-Mecinas M;Gomez-Lima M;Watanabe M;Todd AJ
通讯作者：
Todd AJ
DOI：
10.1126/science.aac8653
发表时间：
2015-10-30
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Bourane S;Duan B;Koch SC;Dalet A;Britz O;Garcia-Campmany L;Kim E;Cheng L;Ghosh A;Ma Q;Goulding M
通讯作者：
Goulding M
影响因子：
64.5
作者：
Duan B;Cheng L;Bourane S;Britz O;Padilla C;Garcia-Campmany L;Krashes M;Knowlton W;Velasquez T;Ren X;Ross S;Lowell BB;Wang Y;Goulding M;Ma Q
通讯作者：
Ma Q
影响因子：
16.2
作者：
Foster, Edmund;Wildner, Hendrik;Tudeau, Laetitia;Haueter, Sabine;Ralvenius, William T.;Jegen, Monika;Johannssen, Helge;Hoesli, Ladina;Haenraets, Karen;Ghanem, Alexander;Conzelmann, Karl-Klaus;Boesl, Michael;Zeilhofer, Hanns Ulrich
通讯作者：
Zeilhofer, Hanns Ulrich
影响因子：
3.3
作者：
Iwagaki N;Garzillo F;Polgár E;Riddell JS;Todd AJ
通讯作者：
Todd AJ